Preclinical TSPO Ligand PET to Visualize Human Glioma Xenotransplants: A Preliminary Study
| Autor: | James P. Harty, Eliot T. McKinley, Lola B. Chambless, Jennifer M. Watchmaker, Reid C. Thompson, Ty W. Abel, H. Charles Manning, Michael K. Cooper, Jason R. Buck, Allie Fu |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Pathology medicine.medical_specialty lcsh:Medicine Biology Sensitivity and Specificity Mice Receptors GABA Neuroimaging Cell Line Tumor Glioma medicine Translocator protein Animals Humans lcsh:Science Multidisciplinary medicine.diagnostic_test Brain Neoplasms lcsh:R Astrocytoma Magnetic resonance imaging medicine.disease Rats 3. Good health Gene Expression Regulation Neoplastic Tissue Array Analysis Positron emission tomography Positron-Emission Tomography biology.protein Biomarker (medicine) Acetanilides lcsh:Q Radiopharmaceuticals Molecular imaging Neoplasm Transplantation Research Article |
| Zdroj: | PLoS ONE, Vol 10, Iss 10, p e0141659 (2015) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Current positron emission tomography (PET) imaging biomarkers for detection of infiltrating gliomas are limited. Translocator protein (TSPO) is a novel and promising biomarker for glioma PET imaging. To validate TSPO as a potential target for molecular imaging of glioma, TSPO expression was assayed in a tumor microarray containing 37 high-grade (III, IV) gliomas. TSPO staining was detected in all tumor specimens. Subsequently, PET imaging was performed with an aryloxyanilide-based TSPO ligand, [18F]PBR06, in primary orthotopic xenograft models of WHO grade III and IV gliomas. Selective uptake of [18F]PBR06 in engrafted tumor was measured. Furthermore, PET imaging with [18F]PBR06 demonstrated infiltrative glioma growth that was undetectable by traditional magnetic resonance imaging (MRI). Preliminary PET with [18F]PBR06 demonstrated a preferential tumor-to-normal background ratio in comparison to 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG). These results suggest that TSPO PET imaging with such high-affinity radiotracers may represent a novel strategy to characterize distinct molecular features of glioma growth, as well as better define the extent of glioma infiltration for therapeutic purposes. |
| Databáze: | OpenAIRE |
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