Kinase and channel activity of TRPM6 are co-ordinated by a dimerization motif and pocket interaction
| Autor: | Helen I. Woodroof, Robert Gourlay, René J. M. Bindels, Maxime G. Blanchard, Thomas Macartney, Jenny van der Wijst, Dario R. Alessi, Joost G. J. Hoenderop |
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| Rok vydání: | 2014 |
| Předmět: |
MBP
myelin basic protein hypomagnesaemia TRPM Cation Channels Protein Serine-Threonine Kinases TRPM transient receptor potential melastatin MAP3K7 Biochemistry MAP2K7 HEK human embryonic kidney Protein structure LDS lithium dodecyl sulfate Humans Magnesium c-Raf Kinase activity Protein kinase A Molecular Biology E embryonic day HSH hypomagnesaemia with secondary hypocalcaemia Hypocalcemia biology phosphorylation Chemistry Cyclin-dependent kinase 2 HRP horseradish peroxidase protein kinase Cell Biology Protein Structure Tertiary 3. Good health Cell biology HEK293 Cells Renal disorders Radboud Institute for Molecular Life Sciences [Radboudumc 11] Protein kinase domain GAPDH glyceraldehyde-3-phosphate dehydrogenase TBST TBS containing Tween 20 ion channel biology.protein transient receptor potential melastatin (TRPM) Protein Multimerization Protein Kinases dimerization motif Research Article |
| Zdroj: | Biochemical Journal, 460, 165-75 Biochemical Journal, 460, 2, pp. 165-75 Biochemical Journal |
| ISSN: | 0264-6021 |
| Popis: | Mutations in the gene that encodes the atypical channel-kinase TRPM6 (transient receptor potential melastatin 6) cause HSH (hypomagnesaemia with secondary hypocalcaemia), a disorder characterized by defective intestinal Mg2+ transport and impaired renal Mg2+ reabsorption. TRPM6, together with its homologue TRPM7, are unique proteins as they combine an ion channel domain with a C-terminally fused protein kinase domain. How TRPM6 channel and kinase activity are linked is unknown. Previous structural analysis revealed that TRPM7 possesses a non-catalytic dimerization motif preceding the kinase domain. This interacts with a dimerization pocket lying within the kinase domain. In the present study, we provide evidence that the dimerization motif in TRPM6 plays a critical role in regulating kinase activity as well as ion channel activity. We identify mutations within the TRPM6 dimerization motif (Leu1718 and Leu1721) or dimerization pocket (L1743A, Q1832K, A1836N, L1840A and L1919Q) that abolish dimerization and establish that these mutations inhibit protein kinase activity. We also demonstrate that kinase activity of a dimerization motif mutant can be restored by addition of a peptide encompassing the dimerization motif. Moreover, we observe that mutations that disrupt the dimerization motif and dimerization pocket interaction greatly diminish TRPM6 ion channel activity, in a manner that is independent of kinase activity. Finally, we analyse the impact on kinase activity of ten disease-causing missense mutations that lie outwith the protein kinase domain of TRPM6. This revealed that one mutation lying nearby the dimerization motif (S1754N), found previously to inhibit channel activity, abolished kinase activity. These results provide the first evidence that there is structural co-ordination between channel and kinase activity, which is mediated by the dimerization motif and pocket interaction. We discuss that modulation of this interaction could comprise a major regulatory mechanism by which TRPM6 function is controlled. We show that TRPM6 kinase activity is linked to channel activity. This occurs through a kinase-independent mechanism involving the dimerization motif binding to a pocket within the kinase domain. A disease-causing mutation (S1754N) lying nearby the dimerization pocket inactivates kinase activity. |
| Databáze: | OpenAIRE |
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