Gut microbial differences in breast and prostate cancer cases from two randomised controlled trials compared to matched cancer-free controls
| Autor: | Tiffany L. Carson, Casey D. Morrow, Wendy Demark-Wahnefried, N E Caston, Andrew D. Frugé, Kristen S Smith, W. Van Der Pol |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Male medicine.medical_specialty Breast Neoplasms Overweight medicine.disease_cause Microbiology Article 03 medical and health sciences Prostate cancer Feces 0302 clinical medicine Breast cancer Prostate Internal medicine Medicine Humans Obesity Randomized Controlled Trials as Topic business.industry Cancer Prostatic Neoplasms Middle Aged medicine.disease Gastrointestinal Microbiome Clinical trial 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Case-Control Studies Female medicine.symptom business Carcinogenesis Body mass index |
| Zdroj: | Benef Microbes |
| ISSN: | 1876-2891 |
| Popis: | Implicated in several chronic diseases, the gastrointestinal microbiome is hypothesised to influence carcinogenesis. We compared faecal microbiota of newly diagnosed treatment-naïve overweight and obese cancer patients and matched controls. Cases were enrolled in presurgical weight-loss trials for breast (NCT02224807) and prostate (NCT01886677) cancers and had a body mass index (BMI) ≥25 kg/m2. Cancer-free controls were matched 1:1 by age (±5 years), race, gender, and BMI (±5 kg/m2). All participants provided faecal samples; isolated bacterial DNA were PCR amplified at the V4 region of the 16S rRNA gene and analysed using the QIIME pipeline. Tests compared cases versus controls, then separately by gender. Microbial alpha-diversity and beta-diversity were assessed, and relative abundance of Operational Taxonomic Units (OTU’s) were compared at the genus level, with false discovery rate (FDR) correction. 22 overweight and obese cancer patients were matched with 22 cancer-free controls, with an average BMI of 30.5±4.3 kg/m2, age 54.4±5.3 years, and 54.5% were black. Fourteen matches were made between breast cancer cases and healthy female controls, and 8 matches were made with prostate cancer cases and healthy male controls. Comparison of all cases and controls revealed no differences in alpha diversity, though prostate cancer patients had higher Chao1 (P=0.006) and Observed Species (P=0.036) than cancer-free males. Beta-diversity metrics were significantly different between cases and controls (P |
| Databáze: | OpenAIRE |
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