α3Na+/K+-ATPase Deficiency Causes Brain Ventricle Dilation and Abrupt Embryonic Motility in Zebrafish
| Autor: | Canan Doganli, Claus Oxvig, Angeles B. Ribera, Karin Lykke-Hartmann, Hans Christian Beck |
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| Rok vydání: | 2013 |
| Předmět: |
Central Nervous System
Proteomics Patch-Clamp Techniques Green Fluorescent Proteins Motility Biochemistry Gene Expression Regulation Enzymologic Cerebral Ventricles Membrane Potentials Animals Genetically Modified Neurobiology ATP1A3 Animals Na+/K+-ATPase Molecular Biology Zebrafish In Situ Hybridization Brain Ventricle Neurons Gene knockdown biology Brain Gene Expression Regulation Developmental Depolarization Cell Biology Basolateral plasma membrane Zebrafish Proteins biology.organism_classification Molecular biology Cell biology Sodium-Potassium-Exchanging ATPase |
| Zdroj: | Doganli, C, Beck, H C, Ribera, A B, Oxvig, C & Lykke-Hartmann, K 2013, ' α3Na+/K+-ATPase deficiency causes brain ventricle dilation and abrupt embryonic motility in zebrafish ', Journal of Biological Chemistry, vol. 288, pp. 8862-8874 . https://doi.org/10.1074/jbc.M112.421529 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m112.421529 |
| Popis: | Na(+)/K(+)-ATPases are transmembrane ion pumps that maintain ion gradients across the basolateral plasma membrane in all animal cells to facilitate essential biological functions. Mutations in the Na(+)/K(+)-ATPase α3 subunit gene (ATP1A3) cause rapid-onset dystonia-parkinsonism, a rare movement disorder characterized by sudden onset of dystonic spasms and slow movements. In the brain, ATP1A3 is principally expressed in neurons. In zebrafish, the transcripts of the two ATP1A3 orthologs, Atp1a3a and Atp1a3b, show distinct expression in the brain. Surprisingly, targeted knockdown of either Atp1a3a or Atp1a3b leads to brain ventricle dilation, a likely consequence of ion imbalances across the plasma membrane that cause accumulation of cerebrospinal fluid in the ventricle. The brain ventricle dilation is accompanied by a depolarization of spinal Rohon-Beard neurons in Atp1a3a knockdown embryos, suggesting impaired neuronal excitability. This is further supported by Atp1a3a or Atp1a3b knockdown results where altered responses to tactile stimuli as well as abnormal motility were observed. Finally, proteomic analysis identified several protein candidates highlighting proteome changes associated with the knockdown of Atp1a3a or Atp1a3b. Our data thus strongly support the role of α3Na(+)/K(+)-ATPase in zebrafish motility and brain development, associating for the first time the α3Na(+)/K(+)-ATPase deficiency with brain ventricle dilation. |
| Databáze: | OpenAIRE |
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