Synthesis, Physicochemical Characterization, and Cytocompatibility of Bioresorbable, Dual-Gelling Injectable Hydrogels
| Autor: | Antonios G. Mikos, Fred K Kasper, Adam K. Ekenseair, Tiffany N. Vo |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Polymers and Plastics
Injectable hydrogels Biological Availability Bioengineering macromolecular substances Article Absorption Cell Line Injections Biomaterials chemistry.chemical_compound Differential scanning calorimetry Tissue engineering Polymer chemistry Materials Chemistry medicine Animals Rheometry technology industry and agriculture Hydrogels Epoxy Rats chemistry Chemical engineering visual_art Self-healing hydrogels Poly(N-isopropylacrylamide) visual_art.visual_art_medium Swelling medicine.symptom |
| Zdroj: | Biomacromolecules. 15:132-142 |
| ISSN: | 1526-4602 1525-7797 |
| DOI: | 10.1021/bm401413c |
| Popis: | Injectable, dual-gelling hydrogels were successfully developed through the combination of physical thermogellation at 37 °C and favorable amine:epoxy chemical cross-linking. Poly(N-isopropylacrylamide)-based thermogelling macromers with a hydrolyzable lactone ring and epoxy pendant groups and a biodegradable diamine-functionalized polyamidoamine cross-linker were synthesized, characterized, and combined to produce nonsyneresing and bioresorbable hydrogels. Differential scanning calorimetry and oscillatory rheometry demonstrated the rapid and dual-gelling nature of the hydrogel formation. The postgelation dimensional stability, swelling, and mechanical behavior of the hydrogel system were shown to be easily tuned in the synthesis and formulation stages. The leachable products were found to be cytocompatible under all conditions, while the degradation products demonstrated a dose- and time-dependent response due to solution osmolality. Preliminary encapsulation studies showed mesenchymal stem cell viability could be maintained for 7 days. The results suggest that injectable and thermally and chemically cross-linkable hydrogels are promising alternatives to prefabricated biomaterials for tissue engineering applications, particularly for cell delivery. |
| Databáze: | OpenAIRE |
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