Oxygen induces fatty acid (n-6)-desaturation independently of temperature inAcanthamoeba castellanii
| Autor: | John L. Harwood, David Lloyd, Andrew J. Rutter, Katie L. Thomas, Marc T. E. Suller |
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| Rok vydání: | 1998 |
| Předmět: |
Fatty Acid Desaturases
Biophysics chemistry.chemical_element Acanthamoeba Biology Biochemistry Oxygen Microsomal fraction chemistry.chemical_compound Structural Biology Microsomes Genetics Protein biosynthesis Animals Flow cytometry Oleate (n-6)-desaturase Molecular Biology Anisomycin chemistry.chemical_classification Acanthamoeba castellanii Degree of unsaturation Temperature Fatty acid Cell Biology Enzyme chemistry Enzyme Induction Limiting oxygen concentration |
| Zdroj: | FEBS Letters. 425:171-174 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(98)00218-x |
| Popis: | Induction of a microsomal oleate delta12 (n-6) desaturase which is mainly responsible for an increase in membrane lipid unsaturation at low temperature has been observed in the free-living amoeba Acanthamoeba castellanii. In this study we show that the enzyme can also be regulated by oxygen independently of temperature in batch cultures grown to O2-limitation. Raising the oxygen concentration from below the lower limit of detection (0.1 microM) to approximately air-saturation (230 microM), whilst maintaining the growth temperature constant (30 degrees C), increased lipid unsaturation and elevated n-6-desaturase activity 2.3-fold. Addition of the protein synthesis inhibitor, anisomycin, showed that increased desaturase activity was due to new protein synthesis rather than activation of pre-existing enzyme. These observations are important for future studies of the mechanism of temperature adaptation in poikilotherms. |
| Databáze: | OpenAIRE |
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