Decoupling the Roles of Cell Shape and Mechanical Stress in Orienting and Cueing Epithelial Mitosis
| Autor: | Oliver E. Jensen, Tobias Starborg, Alexander Nestor-Bergmann, Sarah Woolner, Georgina A. Stooke-Vaughan, Georgina K. Goddard |
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| Přispěvatelé: | Nestor-Bergmann, Alexander [0000-0002-0013-2607], Apollo - University of Cambridge Repository |
| Rok vydání: | 2019 |
| Předmět: |
cell division
Male vertex model Xenopus Spindle Apparatus Article cell shape Stress (mechanics) 03 medical and health sciences Xenopus laevis 0302 clinical medicine Myosin Animals Mitosis lcsh:QH301-705.5 030304 developmental biology mitosis 0303 health sciences biology Cadherin Chemistry mechanical stress Epithelial Cells Decoupling (cosmology) Division (mathematics) Models Theoretical biology.organism_classification cell proliferation Intercellular Junctions lcsh:Biology (General) mitotic spindle Biophysics Female Stress Mechanical epithelium force 030217 neurology & neurosurgery Principal axis theorem |
| Zdroj: | Cell Reports, Vol 26, Iss 8, Pp 2088-2100.e4 (2019) Cell Reports Nestor-Bergmann, A, Stooke-Vaughan, G, Goddard, G, Starborg, T, Jensen, O & Woolner, S 2019, ' Decoupling the roles of cell shape and mechanical stress in orienting and cueing epithelial mitosis ', Cell Reports, vol. 26, no. 8, pp. 2088-2100 . https://doi.org/10.1016/j.celrep.2019.01.102 |
| Popis: | Summary Distinct mechanisms involving cell shape and mechanical force are known to influence the rate and orientation of division in cultured cells. However, uncoupling the impact of shape and force in tissues remains challenging. Combining stretching of Xenopus tissue with mathematical methods of inferring relative mechanical stress, we find separate roles for cell shape and mechanical stress in orienting and cueing division. We demonstrate that division orientation is best predicted by an axis of cell shape defined by the position of tricellular junctions (TCJs), which align with local cell stress rather than tissue-level stress. The alignment of division to cell shape requires functional cadherin and the localization of the spindle orientation protein, LGN, to TCJs but is not sensitive to relative cell stress magnitude. In contrast, proliferation rate is more directly regulated by mechanical stress, being correlated with relative isotropic stress and decoupled from cell shape when myosin II is depleted. Graphical Abstract Highlights • Tissue stretching increases division rate and reorients divisions with stretch • Division orientation is regulated by cell shape defined by tricellular junctions • Cadherin and LGN localize to tricellular junctions aligning division to cell shape • Division rate is linked to mechanical stress and can be decoupled from cell shape Nestor-Bergmann et al. use whole-tissue stretching and mathematical modeling to dissect the roles of mechanical stress and cell shape in cell division. They show that division orientation in stretched tissue is regulated indirectly by changes in cell shape, while division rate is more directly regulated by mechanical stress. |
| Databáze: | OpenAIRE |
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