High sensitivity collisionally-activated decomposition tandem mass spectrometry on a novel quadrupole/orthogonal-acceleration time-of-flight mass spectrometer
| Autor: | Matthias Berg, Howard R. Morris, Robert S. Bordoli, Thanai Paxton, Jean Langhorne, John Hoyes, Anne Dell, Robert Harold Bateman |
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| Rok vydání: | 1996 |
| Předmět: |
Chromatography
Protein mass spectrometry Chemistry Organic Chemistry Selected reaction monitoring Molecular Sequence Data Analytical chemistry Mass spectrometry Top-down proteomics Sample preparation in mass spectrometry Mass Spectrometry Analytical Chemistry Triple quadrupole mass spectrometer Amino Acid Sequence Peptides Quadrupole mass analyzer Spectroscopy Hybrid mass spectrometer |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0951-4198 |
| Popis: | Consideration of the special problems encountered in ultra-high sensitivity biopolymer sequencing studies has led to the development of a novel quadrupole/erthogonal-acceleration time-of-flight tandem mass spectrometer described for the first time here. The performance characteristics of this new geometry are demonstrated, including fully resolved daughter-ion spectra with mass accuracies of 0.1 dalton, which allow removal of interpretation ambiguities and easy differentiation of charge states even in weak collisionally-activated decomposition tandem mass spectra. The instrument has been applied to a variety of biopolymer research problems, including the structure determination of major histocompatibility complex peptide antigens using liquid chromatography/electrospray mass spectrometry and nanoflow-electrospray tandem mass spectrometry, and sequencing capability in the low-femtomole and attomole ranges is demonstrated. |
| Databáze: | OpenAIRE |
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