Automatic cell culture quantitation with TRAKCELL: application to cell toxicology and differentiation
| Autor: | J. C. Bisconte, William Rostène, Santos Carvajal-Gonzalez, G. Colliot, Thierry Caillaud, W. Y. Xu-van Opstal, Claude Billardon |
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| Rok vydání: | 1994 |
| Předmět: |
Tyrosine 3-Monooxygenase
Cell Survival Health Toxicology and Mutagenesis Cellular differentiation Cell Cell Count Ascorbic Acid Biology Toxicology Cell morphology PC12 Cells Dexamethasone chemistry.chemical_compound Mesencephalon Image Processing Computer-Assisted medicine Animals Nerve Growth Factors Rats Wistar Cells Cultured Cell Size Neurons Forskolin Cell growth Colforsin Cell Differentiation Cell Biology Ascorbic acid Culture Media Rats Cell biology medicine.anatomical_structure chemistry Cell culture Immunology Catecholaminergic cell groups Cell Division Software |
| Zdroj: | Cell Biology and Toxicology. 10:387-392 |
| ISSN: | 1573-6822 0742-2091 |
| DOI: | 10.1007/bf00755787 |
| Popis: | An automated system, TRAKCELL, was developed for the quantitation of cells in culture. It enabled cell counting, classification according to morphological cell characteristics and measurement of cell proliferation and differentiation. The system was tested on the toxic effect of ascorbic acid on rat brain catecholaminergic neurons in primary culture. In parallel, the effects of nerve growth factor, dexamethasone and forskolin on cell differentiation were studied using rat pheochromocytoma PC12 cells. The results show that the system permits rapid and reproducible measurements of cell density and of the morphological changes observed following various drug treatments. |
| Databáze: | OpenAIRE |
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