Diagnostic potential of miR-483 family for IGF-II producing non-islet cell tumor hypoglycemia
| Autor: | Akira Asai, Izumi Fukuda, Naomi Hizuka, Lena Eliasson, Hitoshi Sugihara, Mototsugu Nagao, Jonathan L.S. Esguerra |
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| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Blotting Western 030209 endocrinology & metabolism Biology Hypoglycemia Spontaneous hypoglycemia Lesion 03 medical and health sciences 0302 clinical medicine Endocrinology Insulin-Like Growth Factor II Internal medicine Neoplasms microRNA medicine Humans Aged geography geography.geographical_feature_category Growth factor General Medicine Middle Aged medicine.disease Islet Blot MicroRNAs Real-time polymerase chain reaction ROC Curve 030220 oncology & carcinogenesis Area Under Curve Female medicine.symptom |
| Zdroj: | European journal of endocrinology. 184(1) |
| ISSN: | 1479-683X |
| Popis: | Objective In insulin-like growth factor II (IGF-II) producing non-islet cell tumor hypoglycemia (NICTH), high molecular weight forms of IGF-II (big IGF-II) are produced as a cause of spontaneous hypoglycemia. MicroRNA (miRNA)-483 family, encoded in an intron lesion of IGF2 gene, is suggested to be co-expressed with IGF-II. Here, we tested whether serum miR-483-5p and -3p levels are associated with the presence of big IGF-II in NICTH. Design Serum samples from patients who were suspected to have IGF-II producing NICTH (n = 42) were tested. MiR-483-5p and -3p levels were evaluated using quantitative PCR. IGF-II level was analyzed using ELISA. The presence of big IGF-II was identified by Western blotting. Results Big IGF-II was detected in the sera of 32 patients. MiR-483-5p (P = 0.0015) and -3p (P = 0.027) levels were significantly higher in sera with big IGF-II (n = 32) than in those without (n = 10), whereas serum IGF-II level (P = 0.055) was not significantly different between the groups. The median serum concentration of miR-483-5p was ~10 times higher than that of miR-483-3p. Although a strong correlation was observed between the two miRNAs (r = 0.844, P < 0.0001), but neither of which was correlated with serum IGF-II level. The areas under the receiver operating characteristic curves of miR-483-5p (0.853) and -3p (0.722) were higher than that of IGF-II (0.694) for detecting the presence of big IGF-II. Conclusion The associations of serum miR-483-5p and -3p levels with the presence of big IGF-II suggest the diagnostic potential of these miRNAs for IGF-II producing NICTH. |
| Databáze: | OpenAIRE |
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