Biotransformation of Panax ginseng extract by rat intestinal microflora: identification and quantification of metabolites using liquid chromatography-tandem mass spectrometry
| Autor: | Lin-Hu Quan, Wen-Jing Zhu, Jun Jiang, Wei-Wei Dong, Jinhua Zhao, Songquan Wu, Fei-Liang Zhong, Deok-Chun Yang, Donghao Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Metabolite rat intestinal microflora ginsenoside Pharmacology Biochemistry Genetics and Molecular Biology (miscellaneous) complex mixtures 03 medical and health sciences chemistry.chemical_compound Ginseng 0302 clinical medicine GINSENG EXTRACT Biotransformation LC–MS/MS Liquid chromatography–mass spectrometry lcsh:Botany Active metabolite metabolites Chromatography Panax ginseng Primary metabolite food and beverages Metabolism lcsh:QK1-989 030104 developmental biology Complementary and alternative medicine chemistry 030220 oncology & carcinogenesis Biotechnology Research Article |
| Zdroj: | Journal of Ginseng Research Journal of Ginseng Research, Vol 41, Iss 4, Pp 540-547 (2017) |
| ISSN: | 2093-4947 1226-8453 |
| Popis: | Background In general, after Panax ginseng is administered orally, intestinal microbes play a crucial role in its degradation and metabolization process. Studies on the metabolism of P. ginseng by microflora are important for obtaining a better understanding of their biological effects. Methods In vitro biotransformation of P. ginseng extract by rat intestinal microflora was investigated at 37°C for 24 h, and the simultaneous determination of the metabolites and metabolic profile of P. ginseng saponins by rat intestinal microflora was achieved using LC–MS/MS. Results A total of seven ginsenosides were detected in the P. ginseng extract, including ginsenosides Rg1, Re, Rf, Rb1, Rc, Rb2, and Rd. In the transformed P. ginseng samples, considerable amounts of deglycosylated metabolite compound K and Rh1 were detected. In addition, minimal amounts of deglycosylated metabolites (ginsenosides Rg2, F1, F2, Rg3, and protopanaxatriol-type ginsenosides) and untransformed ginsenosides Re, Rg1, and Rd were detected at 24 h. The results indicated that the primary metabolites are compound K and Rh1, and the protopanaxadiol-type ginsenosides were more easily metabolized than protopanaxatriol-type ginsenosides. Conclusion This is the first report of the identification and quantification of the metabolism and metabolic profile of P. ginseng extract in rat intestinal microflora using LC–MS/MS. The current study provided new insights for studying the metabolism and active metabolites of P. ginseng . |
| Databáze: | OpenAIRE |
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