In Vivo Allergen-Activated Eosinophils Promote Collagen I and Fibronectin Gene Expression in Airway Smooth Muscle Cells via TGF-β1 Signaling Pathway in Asthma

Autor: Virginija Kalinauskaite-Zukauske, Kęstutis Malakauskas, Andrius Januškevičius, Ieva Bajoriuniene, Ieva Janulaityte
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Respiratory System
medicine.disease_cause
lcsh:Chemistry
Pathogenesis
Extracellular matrix
extracellular matrix proteins
0302 clinical medicine
Allergen
TGF-β1 signaling
Gene expression
lcsh:QH301-705.5
Spectroscopy
biology
airway smooth muscle cells
General Medicine
respiratory system
Computer Science Applications
medicine.anatomical_structure
030220 oncology & carcinogenesis
Female
bronchial allergen challenge
Signal Transduction
Adult
Myocytes
Smooth Muscle
Article
Bronchial Provocation Tests
Collagen Type I
Catalysis
Transforming Growth Factor beta1
Inorganic Chemistry
Young Adult
03 medical and health sciences
In vivo
fibronectin
medicine
Humans
eosinophil
Physical and Theoretical Chemistry
Molecular Biology
Asthma
collagen I
business.industry
Organic Chemistry
Eosinophil
medicine.disease
TGF- 1 signaling
Fibronectins
respiratory tract diseases
Collagen Type I
alpha 1 Chain
Eosinophils
Fibronectin
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Gene Expression Regulation
Case-Control Studies
Immunology
biology.protein
business
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 5
International journal of molecular sciences, Basel : MDPI, 2020, vol. 21, no. 5, E1837, p. 1-19
International Journal of Molecular Sciences, Vol 21, Iss 5, p 1837 (2020)
ISSN: 1422-0067
DOI: 10.3390/ijms21051837
Popis: Eosinophils infiltration and releasing TGF-&beta
1 in the airways has been implicated in the pathogenesis of asthma, especially during acute episodes provoked by an allergen. TGF-&beta
1 is a major mediator involved in pro-inflammatory responses and fibrotic tissue remodeling in asthma. We aimed to evaluate the effect of in vivo allergen-activated eosinophils on the expression of COL1A1 and FN in ASM cells in asthma. A total of 12 allergic asthma patients and 11 healthy subjects were examined. All study subjects underwent bronchial challenge with D. pteronyssinus allergen. Eosinophils from peripheral blood were isolated before and 24 h after the bronchial allergen challenge using high-density centrifugation and magnetic separation. Individual co-cultures of blood eosinophils and immortalized human ASM cells were prepared. The TGF-&beta
1 concentration in culture supernatants was analyzed using ELISA. Gene expression was analyzed using qRT-PCR. Eosinophils integrins were suppressed with linear RGDS peptide before co-culture with ASM cells. Results: The expression of TGF-&beta
1 in asthmatic eosinophils significantly increased over non-activated asthmatic eosinophils after allergen challenge, p <
0.001. The TGF-&beta
1 concentration in culture supernatants was significantly higher in samples with allergen-activated asthmatic eosinophils compared to baseline, p <
0.05. The effect of allergen-activated asthmatic eosinophils on the expression of TGF-&beta
1, COL1A1, and FN in ASM cells was more significant compared to non-activated eosinophils, p <
0.05, however, no difference was found on WNT-5A expression. The incubation of allergen-activated asthmatic eosinophils with RGDS peptide was more effective compared to non-activated eosinophils as the gene expression in ASM cells was downregulated equally to the same level as healthy eosinophils.
Databáze: OpenAIRE
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