Karrikin Signaling Acts Parallel to and Additively with Strigolactone Signaling to Regulate Rice Mesocotyl Elongation in Darkness
| Autor: | Jiayang Li, Qian Qian, Shujing Kang, Jiarong Dai, Linyuan Zou, Dali Zeng, Jianshu Zheng, Minghao Qu, Lixin Zhu, Zhanpeng Tang, Jiang Hu, Peng Cui, Kai Hong, Guosheng Xiong, Quan Wang, Bing Wang, Xiangbing Meng, Lei Wang, Yonghui Zhao, Longjun Zeng, Yonghong Wang |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Strigolactone Plant Science Biology 01 natural sciences Lactones 03 medical and health sciences Ubiquitin Gene Expression Regulation Plant Gene expression Furans Research Articles Plant Proteins Pyrans Regulation of gene expression Ubiquitination food and beverages Oryza Stereoisomerism Cell Biology Darkness Plants Genetically Modified Cell biology Karrikin Ubiquitin ligase 030104 developmental biology biology.protein Elongation Signal transduction Heterocyclic Compounds 3-Ring Plant Shoots Signal Transduction 010606 plant biology & botany |
| Zdroj: | Plant Cell |
| ISSN: | 1532-298X 1040-4651 |
| Popis: | Seedling emergence in monocots depends mainly on mesocotyl elongation, requiring coordination between developmental signals and environmental stimuli. Strigolactones (SLs) and karrikins are butenolide compounds that regulate various developmental processes; both are able to negatively regulate rice (Oryza sativa) mesocotyl elongation in the dark. Here, we report that a karrikin signaling complex, DWARF14-LIKE (D14L)-DWARF3 (D3)-O. sativa SUPPRESSOR OF MAX2 1 (OsSMAX1) mediates the regulation of rice mesocotyl elongation in the dark. We demonstrate that D14L recognizes the karrikin signal and recruits the SCF(D3) ubiquitin ligase for the ubiquitination and degradation of OsSMAX1, mirroring the SL-induced and D14- and D3-dependent ubiquitination and degradation of D53. Overexpression of OsSMAX1 promoted mesocotyl elongation in the dark, whereas knockout of OsSMAX1 suppressed the elongated-mesocotyl phenotypes of d14l and d3. OsSMAX1 localizes to the nucleus and interacts with TOPLESS-RELATED PROTEINs, regulating downstream gene expression. Moreover, we showed that the GR24 enantiomers GR24(5DS) and GR24(ent-5DS) specifically inhibit mesocotyl elongation and regulate downstream gene expression in a D14- and D14L-dependent manner, respectively. Our work revealed that karrikin and SL signaling play parallel and additive roles in modulating downstream gene expression and negatively regulating mesocotyl elongation in the dark. |
| Databáze: | OpenAIRE |
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