Mesothelial cells: A cellular surrogate for tissue engineering of corneal endothelium
| Autor: | Bernat Soria, Felipe Soria, Natalia Escacena, Jorge L. Alió, Elena Quesada-Hernández, Christian Claude Lachaud, Abdelkrim Hmadcha |
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| Přispěvatelé: | European Commission, Fundación Progreso y Salud, Junta de Andalucía, Ministerio de Ciencia e Innovación (España), Red de Terapia Celular (España), Instituto de Salud Carlos III, Ministerio de Sanidad y Consumo (España) |
| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty Corneal endothelium Primary Cell Culture Lens Capsule Crystalline Adipose tissue Mice Inbred Strains Intra-Abdominal Fat Epithelium Corneal Diseases Mice Microscopy Electron Transmission Animals Humans Medicine Mesothelial cells Tissue Engineering business.industry Endothelium Corneal Electronic microscope Epithelial Cells Adult Stem Cells Female Christian ministry Lens capsules business Humanities Biomarkers |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | [Purpose]: To evaluate whether mouse adipose tissue mesothelial cells (ATMCs) share morphologic and biochemical characteristics with mouse corneal endothelial cells (CECs) and to evaluate their capacity to adhere to the decellularized basal membrane of human anterior lens capsules (HALCs) as a potential tissue-engineered surrogate for corneal endothelium replacement. [Methods]: Adipose tissue mesothelial cells were isolated from the visceral adipose tissue of adult mice, and their expression of several corneal endothelium markers was determined with quantitative RT-PCR, immunofluorescence, and Western blotting. Adipose tissue mesothelial cells were cultured in a mesothelial retaining phenotype medium (MRPM) and further seeded and cultured on top of the decellularized basal membrane of HALCs. ATMC-HALC composites were evaluated by optical microscopy, immunofluorescence, and transmission electron microscopy. [Results]: Mesothelial retaining phenotype medium-cultured ATMCs express the corneal endothelium markers COL4A2, COL8A2, SLC4A4, CAR2, sodium- and potassium-dependent adenosine triphosphatase (Na+/K+-ATPase), b-catenin, zona occludens-1, and N-cadherin in a pattern similar to that in mouse CECs. Furthermore, ATMCs displayed strong adhesion capacity onto the basal membrane of HALCs and formed a confluent monolayer within 72 hours of culture in MRPM. Ultrastructural morphologic and marker characteristics displayed by ATMC monolayer on HALCs clearly indicated that ATMCs retained their original phenotype of squamous epithelial-like cells. [Conclusions]: Corneal epithelial cells and ATMCs share morphologic (structural) and marker (functional) similarities. The ATMCs adhered and formed structures mimicking focal adhesion complexes with the HALC basal membrane. Monolayer structure and achieved density of ATMCs support the proposal to use adult human mesothelial cells (MCs) as a possible surrogate for damaged corneal endothelium. Supported by Fondos FEDER, Fundación Progreso y Salud, Consejería de Salud, Junta de Andalucía (Grant PI-0022/2008), INNPACTO Program (INP-2011-1615-900000), and SUDOE Program-BIOREG (Intereg SOE3/P1/E750); Consejer´ıa de Innovación Ciencia y Empresa, Junta de Andalucía (Grant CTS-6505); Ministry of Science and Innovation (Red TerCel-FEDER Grant RD12/0019/ 0028); Instituto de Salud Carlos III Grant PI10/00964); the Ministry of Health and Consumer Affairs Advanced Therapies Program Grant TRA-120 (BS); and Corporación Tecnológica de Andalucía CTA (NBT). CIBERDEM is an initiative of the Instituto de Salud Carlos III. |
| Databáze: | OpenAIRE |
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