Epigenetic regulation of Myc on retinoic acid receptor beta and PDLIM4 in RWPE1 cells
| Autor: | R. Jeffrey Karnes, Donkena Krishna Vanaja, Meilan He, Charles Y.F. Young |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Receptors Retinoic Acid Urology Blotting Western DNMT3B Genes myc Retinoic acid receptor beta Cell Growth Processes Biology Transfection Polymerase Chain Reaction DNA methyltransferase Cell Line DNA Methyltransferase 3A Epigenesis Genetic Prostate cancer medicine Humans DNA (Cytosine-5-)-Methyltransferases Epigenetics Gene Expression Profiling Prostate Prostatic Neoplasms DNA Sequence Analysis DNA Methylation DNA Methylation LIM Domain Proteins medicine.disease DNA-Binding Proteins Oncology CpG site DNA methylation Cancer research |
| Zdroj: | The Prostate. 69:1643-1650 |
| ISSN: | 1097-0045 0270-4137 |
| DOI: | 10.1002/pros.21013 |
| Popis: | BACKGROUND Hypermethylation of CpG islands is a common epigenetic alteration associated with cancer. Tumor suppressor genes retinoic acid receptor beta (RARβ) and PDLIM4 are hypermethylated and silenced in prostate cancer (PCa) tissues and PCa cell lines compared to normal prostate cells. METHODS In this study, a benign prostate epithelial cell line RWPE1 was used as a model to study the epigenetic regulation of Myc on the RARβ and PDLIM4 promoters. Forced Myc overexpression inhibited the RARβ and PDLIM4 expression. RESULTS Pyrosequencing study showed that Myc overexpression increased methylation in several CpG sites of both promoters. A DNA methylation inhibitor 5-aza-2′-deoxycytidine reversed the epigenetic alteration effect of Myc on both RARβ and PDLIM4. CONCLUSION The epigenetic regulation of Myc may be related to its up-regulation of the DNA methyltransferase DNMT3a and DNMT3b. Prostate 69: 1643–1650, 2009. © 2009 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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