Analyses of a Bacillus subtilis homologue of the Na+/H+ antiporter gene which is important for pH homeostasis of alkaliphilic Bacillus sp. C-125
| Autor: | Saori Kosono, Toshiaki Kudo, Makio Kitada, Shinya Morotomi |
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| Rok vydání: | 1999 |
| Předmět: |
Sodium-Hydrogen Exchangers
(Bacillus subtilis) Antiporter Biophysics Bacillus subtilis Biology Genome Biochemistry chemistry.chemical_compound Homeostasis Gene Na+/H+ antiporter Membrane potential Strain (chemistry) Na+ transporter pH homeostasis Cell Biology Hydrogen-Ion Concentration biology.organism_classification Molecular biology chemistry Mutagenesis Multigene Family DNA |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1409(3):171-175 |
| ISSN: | 0005-2728 |
| DOI: | 10.1016/s0005-2728(98)00157-1 |
| Popis: | Bacillus subtilis was revealed to have a homologous region to the DNA fragment responsible for alkaliphily of alkaliphilic Bacillus sp. C-125 on the genome, as reported previously [1] . The yufT gene on the B. subtilis genome showed a significant similarity with ORF1 of Bacillus sp. C-125, which is related to membrane potential (ΔΨ)-driven Na+/H+ antiport activity and is important for pH homeostasis in an alkaline condition. Disruption of the yufT gene resulted in the decrease of Na+/H+ antiport activity, and the growth of the yufT disrupted strain was impaired with an increase in the external Na+ concentration. We conclude that the yufT gene encodes a Na+/H+ antiporter, which has a dominant role in the extrusion of cytotoxic Na+. |
| Databáze: | OpenAIRE |
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