Comparison of gyrB gene sequences, 16S rRNA gene sequences and DNA–DNA hybridization in the Bacillus subtilis group
| Autor: | Fwu-Ling Lee, Chun-Ju Tai, Li-Ting Wang, Hiroaki Kasai |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
DNA
Bacterial Genetics Phylogenetic tree Sequence analysis DNA–DNA hybridization Molecular Sequence Data Nucleic acid sequence Nucleic Acid Hybridization Sequence Analysis DNA General Medicine Biology Ribosomal RNA DNA Ribosomal Microbiology Nucleic acid thermodynamics DNA Gyrase RNA Ribosomal 16S Gene Peptide sequence Phylogeny Ecology Evolution Behavior and Systematics Bacillus subtilis |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 57:1846-1850 |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | TheBacillus subtilisgroup comprises eight closely related species that are indistinguishable from one another by 16S rRNA gene sequence analysis. Therefore, thegyrBgene, which encodes the subunit B protein of DNA gyrase, was selected as an alternative phylogenetic marker. To determine whethergyrBgene sequence analysis could be used for phylogenetic analysis and species identification of members of theB. subtilisgroup, the congruence ofgyrBgrouping with both 16S rRNA gene sequencing and DNA–DNA hybridization data was evaluated. Ranges ofgyrBnucleotide and translated amino acid sequence similarities among the eight type strains were 75.4–95.0 % and 88.5–99.2 %, respectively, whereas 16S rRNA gene sequence similarities were 98.1–99.8 %. Results showed thatgyrBgene sequences provide higher resolution than 16S rRNA gene sequences. The classification achieved bygyrBsequence analysis was in agreement with results obtained with DNA–DNA hybridization. It is concluded that thegyrBgene may be an efficient alternative target for identification and taxonomic analysis of members of theB. subtilisgroup. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|