An In Vitro Comparative Study of the Effects of Tetrabromobisphenol A and Tetrabromobisphenol S on Human Erythrocyte Membranes—Changes in ATP Level, Perturbations in Membrane Fluidity, Alterations in Conformational State and Damage to Proteins
| Autor: | Bogumiła Huras, Monika Jarosiewicz, Paweł Jarosiewicz, Piotr Duchnowicz, Bożena Bukowska |
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| Rok vydání: | 2021 |
| Předmět: |
Erythrocytes
QH301-705.5 Membrane Fluidity Protein Conformation Polybrominated Biphenyls Article Catalysis Inorganic Chemistry Lipid peroxidation chemistry.chemical_compound Adenosine Triphosphate Membrane fluidity Humans Biology (General) Physical and Theoretical Chemistry QD1-999 tetrabromobisphenol A Molecular Biology Spectroscopy Flame Retardants Erythrocyte Membrane Organic Chemistry Membrane Proteins Proteins General Medicine tetrabromobisphenol S retardants Healthy Volunteers In vitro Computer Science Applications Chemistry Membrane chemistry Membrane protein Toxicity Biophysics Tetrabromobisphenol A Xenobiotic |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 9443, p 9443 (2021) Volume 22 Issue 17 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22179443 |
| Popis: | Brominated flame retardants (BFRs) are substances used to reduce the flammability of plastics. Among this group, tetrabormobisphenol A (TBBPA) is currently produced and used on the greatest scale, but due to the emerging reports on its potential toxicity, tetrabromobisphenol S (TBBPS)—a compound with a very similar structure—is used as an alternative. Due to the fact that the compounds in question are found in the environment and in biological samples from living organisms, including humans, and due to the insufficient toxicological knowledge about them, it is necessary to assess their impacts on living organisms and verify the validity of TBBPA replacement by TBBPS. The RBC membrane was chosen as the research model. This is a widely accepted research model for assessing the toxicity of xenobiotics, and it is the first barrier to compounds entering circulation. It was found that TBBPA and TBBPS caused increases in the fluidity of the erythrocyte membrane in their hydrophilic layer, and conformational changes to membrane proteins. They also caused thiol group elevation, an increase in lipid peroxidation (TBBPS only) and decreases in the level of ATP in cells. They also caused changes in the size and shape of RBCs. TBBPA caused changes in the erythrocyte membrane at lower concentrations compared to TBBPS at an occupational exposure level. |
| Databáze: | OpenAIRE |
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