OMV-based vaccine formulations against Shiga toxin producing Escherichia coli strains are both protective in mice and immunogenic in calves
| Autor: | Mirta Lescano, José Christian Dokmetjian, Luciana Vázquez, Matías Fingermann, Lucía Ávila, José Luis Pérez Quiñoy, Andrea Verónica Müller, Darío Nicolás Di Biase, Sonsire Fernández Castillo, Maria Belén De Marco |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Serotype Drug Compounding Immunology Escherichia coli Vaccines Lethal challenge Short Report Virulence Cattle Diseases Hemolytic Uremic Syndrome medicine.disease_cause Microbiology 03 medical and health sciences Zoonosis Cell-Derived Microparticles medicine Immunology and Allergy Animals Escherichia coli Escherichia coli Infections Pharmacology Mice Inbred BALB C biology Shiga-Toxigenic Escherichia coli Shiga toxin Outer Membrane Vesicles medicine.disease biology.organism_classification 030104 developmental biology Models Animal biology.protein Cattle Bacterial outer membrane Vaccine Bacteria |
| Zdroj: | Human Vaccines & Immunotherapeutics |
| ISSN: | 2164-554X 2164-5515 |
| Popis: | Strains of Shiga toxin-producing Escherichia coli (STEC) can cause the severe Hemolytic Uremic Syndrome (HUS). Shiga toxins are protein toxins that bind and kill microvascular cells, damaging vital organs. No specific therapeutics or vaccines have been licensed for use in humans yet. The most common route of infection is by consumption of dairy or farm products contaminated with STEC. Domestic cattle colonized by STEC strains represent the main reservoir, and thus a source of contamination. Outer Membrane Vesicles (OMV) obtained after detergent treatment of gram-negative bacteria have been used over the past decades for producing many licensed vaccines. These nanoparticles are not only multi-antigenic in nature but also potent immunopotentiators and immunomodulators. Formulations based on chemical-inactivated OMV (OMVi) obtained from a virulent STEC strain (O157:H7 serotype) were found to protect against pathogenicity in a murine model and to be immunogenic in calves. These initial studies suggest that STEC-derived OMV has a potential for the formulation of both human and veterinary vaccines. |
| Databáze: | OpenAIRE |
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