High-speed, cortex-wide volumetric recording of neuroactivity at cellular resolution using light beads microscopy

Autor: Hyewon Kim, Frank Tejera, Jeff Demas, Kevin Barber, Brandon Chen, Francisca Martínez Traub, Jason Manley, Alipasha Vaziri
Rok vydání: 2021
Předmět:
Zdroj: Nat Methods
ISSN: 1548-7105
1548-7091
DOI: 10.1038/s41592-021-01239-8
Popis: Two-photon microscopy has enabled high-resolution imaging of neuroactivity at depth within scattering brain tissue. However, its various realizations have not overcome the tradeoffs between speed and spatiotemporal sampling that would be necessary to enable mesoscale volumetric recording of neuroactivity at cellular resolution and speed compatible with resolving calcium transients. Here, we introduce light beads microscopy (LBM), a scalable and spatiotemporally optimal acquisition approach limited only by fluorescence lifetime, where a set of axially separated and temporally distinct foci record the entire axial imaging range near-simultaneously, enabling volumetric recording at 1.41 × 108 voxels per second. Using LBM, we demonstrate mesoscopic and volumetric imaging at multiple scales in the mouse cortex, including cellular-resolution recordings within ~3 × 5 × 0.5 mm volumes containing >200,000 neurons at ~5 Hz and recordings of populations of ~1 million neurons within ~5.4 × 6 × 0.5 mm volumes at ~2 Hz, as well as higher speed (9.6 Hz) subcellular-resolution volumetric recordings. LBM provides an opportunity for discovering the neurocomputations underlying cortex-wide encoding and processing of information in the mammalian brain. Light beads microscopy is a two-photon microscopy approach that allows high-speed volumetric imaging of neuronal activity at the mesoscale.
Databáze: OpenAIRE
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