Overexpression approaches to advance understanding of Candida albicans

Autor: Sophie Bachellier-Bassi, Lasse van Wijlick, Melanie Legrand, Murielle Chauvel, Laxmi Shanker Rai, Christophe d'Enfert
Přispěvatelé: Biologie et Pathogénicité fongiques - Fungal Biology and Pathogenicity (BPF), Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), LSR is the recipient of a post-doctoral fellowship of the Fondation pour la Recherche Médicale (FRM, DBF20160635719) and LvW of the Agence Nationale de la Recherche (CANDIHUB, ANR-14-CE-0018). We also acknowledge Neha Varshney from Ludwig Institute of Cancer Research, San Diego for her help in making the figure. Work in the d’Enfert laboratory aimed at developing and exploiting overexpression approaches in C. albicans has been supported by the European Commission (EURESFUN, LSHM-CT-2005-518199, Galar Fungail 2, MRTN-CT-2003-504148, FINSysB, PITN-GA-2008-214004), Agence Nationale de la Recherche (KANJI, ANR-08-MIE-033-01, CANDIHUB, ANR-14-CE-0018, FunComPath ANR-14-IFEC-0004), the Wellcome Trust (The Candida albicans ORFeome project, WT088858MA), Fondation de la Recherche Médicale (DBF20160635719), the French Government's Investissement d'Avenir program, (Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases', ANR-10-LABX-62-IBEID, Institut de Recherche Technologique BIOASTER, ANR-10-AIRT-03), Institut Pasteur (Bourse Roux to M. Legrand), and the Indo-French Centre for the Promotion of Advanced Research (CEFIPRA)., ANR-14-IFEC-0004,FunComPath,From fungal commensalism to pathogenicity:dissection of the colonization-to-infection shift of Candida albicans(2014), ANR-14-CE14-0018,CANDIHUB,Réponse au stress et virulence chez les champignons pathogènes de l'homme : une approche comparative systémique des réseaux de régulation chez les espèces du genre Candida(2014), ANR-08-MIEN-0033,KANJI,Colonisation et invasion de la muqueuse digestive par le champignon pathogène de l'homme Candida albicans(2008), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-10-AIRT-0003,BIOASTER,BIOASTER(2010), European Project: 32889,EURESFUN
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Molecular Microbiology
Molecular Microbiology, 2022, 117 (3), pp.589-599. ⟨10.1111/mmi.14818⟩
ISSN: 0950-382X
1365-2958
Popis: International audience; Candida albicans is an opportunistic fungal pathogen that is responsible for infections linked to high mortality. Loss-of-function approaches, taking advantage of gene knockouts or inducible down-regulation, have been successfully used in this species in order to understand gene function. However, overexpression of a gene provides an alternative, powerful tool to elucidate gene function and identify novel phenotypes. Notably, overexpression can identify pathway components that might remain undetected using loss-of-function approaches. Several repressible or inducible promoters have been developed which allow to shut off or turn on the expression of a gene in C. albicans upon growth in the presence of a repressor or inducer. In this review, we summarize recent overexpression approaches used to study different aspects of C. albicans biology, including morphogenesis, biofilm formation, drug tolerance, and commensalism.
Databáze: OpenAIRE
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