Trans-splicing with the group I intron ribozyme from Azoarcus
| Autor: | Ulrich Müller, Gregory F. Dolan |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Molecular Sequence Data Azoarcus ComputerApplications_COMPUTERSINOTHERSYSTEMS Substrate Specificity Tetrahymena thermophila Trans-Splicing Escherichia coli RNA Catalytic RNA Messenger Molecular Biology Ligase ribozyme biology Base Sequence GIR1 branching ribozyme Inverted Repeat Sequences Ribozyme Articles biology.organism_classification GlmS glucosamine-6-phosphate activated ribozyme RNA Bacterial Biochemistry biology.protein Mammalian CPEB3 ribozyme Hairpin ribozyme VS ribozyme RNA Protozoan |
| Zdroj: | RNA (New York, N.Y.). 20(2) |
| ISSN: | 1469-9001 |
| Popis: | Group I introns are ribozymes (catalytic RNAs) that excise themselves from RNA primary transcripts by catalyzing two successive transesterification reactions. These cis-splicing ribozymes can be converted into trans-splicing ribozymes, which can modify the sequence of a separate substrate RNA, both in vitro and in vivo. Previous work on trans-splicing ribozymes has mostly focused on the 16S rRNA group I intron ribozyme from Tetrahymena thermophila. Here, we test the trans-splicing potential of the tRNAIle group I intron ribozyme from the bacterium Azoarcus. This ribozyme is only half the size of the Tetrahymena ribozyme and folds faster into its active conformation in vitro. Our results showed that in vitro, the Azoarcus and Tetrahymena ribozymes favored the same set of splice sites on a substrate RNA. Both ribozymes showed the same trans-splicing efficiency when containing their individually optimized 5′ terminus. In contrast to the previously optimized 5′-terminal design of the Tetrahymena ribozyme, the Azoarcus ribozyme was most efficient with a trans-splicing design that resembled the secondary structure context of the natural cis-splicing Azoarcus ribozyme, which includes base-pairing between the substrate 5′ portion and the ribozyme 3′ exon. These results suggested preferred trans-splicing interactions for the Azoarcus ribozyme under near-physiological in vitro conditions. Despite the high activity in vitro, however, the splicing efficiency of the Azoarcus ribozyme in Escherichia coli cells was significantly below that of the Tetrahymena ribozyme. |
| Databáze: | OpenAIRE |
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