AVR2 Targets BSL Family Members, Which Act as Susceptibility Factors to Suppress Host Immunity
| Autor: | Haixia Wang, Susan Breen, Lydia Welsh, Lina Yang, Marek Malec, Piers A. Hemsley, Eleanor M. Gilroy, Paul R. J. Birch, Tian Zhendong, Shaista Naqvi, Dionne Turnbull, Frédéric Brunner |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Phytophthora infestans Virulence Factors Physiology Plant Immunity Virulence Plant Science Biology 01 natural sciences Immune system Immunity Tobacco Signaling and Response Genetics Gene silencing Gene Silencing Plant Diseases Plant Proteins Solanum tuberosum Effector fungi food and beverages Plants Genetically Modified biology.organism_classification Plant disease Host-Pathogen Interactions 010606 plant biology & botany |
| Zdroj: | Plant Physiology. 180:571-581 |
| ISSN: | 1532-2548 0032-0889 |
| Popis: | To be successful plant pathogens, microbes use “effector proteins” to manipulate host functions to their benefit. Identifying host targets of effector proteins and characterizing their role in the infection process allow us to better understand plant–pathogen interactions and the plant immune system. Yeast two-hybrid analysis and coimmunoprecipitation were used to demonstrate that the Phytophthora infestans effector AVIRULENCE 2 (PiAVR2) interacts with all three BRI1-SUPPRESSOR1-like (BSL) family members from potato (Solanum tuberosum). Transient expression of BSL1, BSL2, and BSL3 enhanced P. infestans leaf infection. BSL1 and BSL3 suppressed INFESTIN 1 elicitin-triggered cell death, showing that they negatively regulate immunity. Virus-induced gene silencing studies revealed that BSL2 and BSL3 are required for BSL1 stability and show that basal levels of immunity are increased in BSL-silenced plants. Immune suppression by BSL family members is dependent on the brassinosteroid-responsive host transcription factor CIB1/HBI1-like 1. The P. infestans effector PiAVR2 targets all three BSL family members in the crop plant S. tuberosum. These phosphatases, known for their role in growth-promoting brassinosteroid signaling, all support P. infestans virulence and thus can be regarded as susceptibility factors in late blight infection. |
| Databáze: | OpenAIRE |
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