The Synchytrium endobioticum AvrSen1 triggers a Hypersensitive Response in Sen1 potatoes while natural variants evade detection
| Autor: | Theo van der Lee, Richard G. F. Visser, Charlotte Prodhomme, Marga P. E. van Gent-Pelzer, Marjan Bergervoet, Gert van Arkel, Jarosław Przetakiewicz, Balázs Brankovics, Bart T. L. H. van de Vossenberg, Jack H. Vossen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hypersensitive response Candidate gene hypersensitive response Synchytrium endobioticum Physiology chytridiomycota Select agent 01 natural sciences Biointeractions and Plant Health 03 medical and health sciences Laboratorium voor Plantenveredeling proteomics Pathogen Gene Genetics avirulence factors Chytridiomycota biology Effector resistance genes plant-pathogen interactions fungus-plant interactions population genetics General Medicine Genomics PE&RC biology.organism_classification metabolomics Plant Breeding 030104 developmental biology cell death effector EPS Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Molecular Plant-Microbe Interactions 32 (2019) 11 Molecular Plant-Microbe Interactions, 32(11), 1536-1546 |
| ISSN: | 0894-0282 |
| Popis: | Synchytrium endobioticum is an obligate biotrophic fungus of division Chytridiomycota. It causes potato wart disease, has a worldwide quarantine status and is included on the Health and Human Services and United States Department of Agriculture Select Agent list. S. endobioticum isolates are grouped in pathotypes based on their ability to evade host resistance in a set of differential potato varieties. Thus far, 39 pathotypes are reported. A single dominant gene (Sen1) governs pathotype 1 (D1) resistance and we anticipated that the underlying molecular model would involve a pathogen effector (AvrSen1) that is recognized by the host. The S. endobioticum-specific secretome of 14 isolates representing six different pathotypes was screened for effectors specifically present in pathotype 1 (D1) isolates but absent in others. We identified a single AvrSen1 candidate. Expression of this candidate in potato Sen1 plants showed a specific hypersensitive response (HR), which cosegregated with the Sen1 resistance in potato populations. No HR was obtained with truncated genes found in pathotypes that evaded recognition by Sen1. These findings established that our candidate gene was indeed Avrsen1. The S. endobioticum AvrSen1 is a single-copy gene and encodes a 376-amino-acid protein without predicted function or functional domains, and is the first effector gene identified in Chytridiomycota, an extremely diverse yet underrepresented basal lineage of fungi. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|