Characterization of Polyhydroxyalkanoate Produced by Bacillus megaterium VB89 Isolated from Nisargruna Biogas Plant
| Autor: | R.L. Deopurkar, Ashwini Rane, Vishakha V. Baikar |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Dispersity Bioengineering 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Polyhydroxyalkanoates Polyhydroxybutyrate 03 medical and health sciences 010608 biotechnology Polymer chemistry Thermal stability Molecular Biology Bacillus megaterium biology Chemistry Thermal decomposition technology industry and agriculture General Medicine biology.organism_classification Biodegradable polymer 030104 developmental biology Chemical engineering lipids (amino acids peptides and proteins) Glass transition Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 183:241-253 |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/s12010-017-2441-4 |
| Popis: | Polyhydroxyalkanoates (PHAs) are naturally occurring biodegradable polymers that can curb the extensive use of polypropylene based plastics. In contrast to chemically synthesized polypropylene plastics, PHAs are biodegradable and thus environmentally safe. PHAs have attracted much attention as biocompatible and biodegradable thermoplastics. The present study involves isolation of bacteria from different environments capable of synthesizing PHAs. The bacterium producing highest yield of PHA (0.672 ± 0.041 g/L) was identified as Bacillus megaterium VB89 by biochemical and molecular techniques such as 16S rDNA sequence analysis. Strain VB89 produced polyhydroxybutyrate (PHB) as revealed by FTIR and NMR. This PHB had an average molecular weight of 2.89 × 105 Da and a polydispersity index of 2.37. Thermal properties of the PHB included a glass transition temperature of 13.97 °C, a melting temperature of 181.74 °C, and a decomposition temperature of 234 °C. All these properties indicated that VB89 produced PHB of high purity and good thermal stability. |
| Databáze: | OpenAIRE |
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