Effect of monensin on fluid phase and receptor mediated endocytosis by rat hepatocyte monolayers
| Autor: | Jacqueline Thirion, Robert Wattiaux |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Sucrose
animal structures Endosome Dipeptidyl Peptidase 4 Biophysics Asialoglycoproteins Golgi Apparatus Biology Endocytosis Biochemistry chemistry.chemical_compound medicine Animals Monensin Dipeptidyl-Peptidases and Tripeptidyl-Peptidases Fetuins Molecular Biology Galactosyltransferase Differential centrifugation Cell Biology Receptor-mediated endocytosis Galactosyltransferases Rats medicine.anatomical_structure chemistry Liver Hepatocyte alpha-Fetoproteins Lysosomes Intracellular |
| Zdroj: | Biochemical and biophysical research communications. 152(3) |
| ISSN: | 0006-291X |
| Popis: | We have investigated the effect of monensin on the uptake of sucrose and sucrose-asialofetuin by rat hepatocyte monolayers and on the intracellular traffic of these molecules. Endocytosis of sucrose is not affected by monensin while that of sucrose-asialofetuin is markedly inhibited. Monensin causes a decrease of galactosyltransferase, a Golgi membrane enzyme without affecting dipeptidylpeptidase IV a plasma membrane enzyme and three lysosomal hydrolases. The inhibition of sucrose-asialofetuin uptake is proportional to the reduction of galactosyltransferase activity. Differential and isopycnic centrifugation results indicate that monensin at a concentration (10 mumol/l) that markedly decreases sucrose-asialofetuin uptake does not prevent the transfer of sucrose and sucrose-asialofetuin from endosomes to lysosomes. |
| Databáze: | OpenAIRE |
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