Hexyl-aminolevulinate ethosome-mediated photodynamic therapy against acne: in vitro and in vivo analyses
| Autor: | Tai Wang, Jinru Song, Xiaoliang Zhu, Lifang Wu, Yingzhe Wang, Feiyin Zhang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_treatment
Pharmaceutical Science Inflammation Photodynamic therapy 02 engineering and technology Pharmacology 030226 pharmacology & pharmacy Rats Sprague-Dawley 03 medical and health sciences Propionibacterium acnes 0302 clinical medicine In vivo Acne Vulgaris medicine Animals Acne Photosensitizing Agents biology business.industry Therapeutic effect Histology Aminolevulinic Acid 021001 nanoscience & nanotechnology biology.organism_classification medicine.disease In vitro Rats Photochemotherapy medicine.symptom 0210 nano-technology business |
| Zdroj: | Drug delivery and translational research. 12(1) |
| ISSN: | 2190-3948 |
| Popis: | Biofilm formation by Propionibacterium acnes is known to cause failure of anti-acne treatment. Conventional therapies for acne are typically inadequate. Accordingly, in this study, we evaluated the therapeutic potential of photodynamic therapy (PDT) using hexyl-aminolevulinate (HAL)-loaded ethosomes (ESs) against the biofilms of P. acnes in vitro and P. acnes–induced inflammatory acne model in vivo. The antibacterial effects of HAL ESs were evaluated using XTT colorimetric assays and scanning electron microscopic observations of morphological changes. P. acnes was intradermally injected into the ears of Sprague-Dawley rats, and the anti-inflammatory effects of HAL ESs were measured by determining changes in appearance, histology, and the antibacterial effects by P. acnes abundance in ear tissues compared with blank control ESs, HAL alone, and 5-aminolevulinic acid (ALA) alone. The highest reduction in viability in P. acnes biofilms was observed after treatment with 5 mg/mL HAL ESs. Notably, blank control ESs also showed significant inhibitory effects. Furthermore, HAL ESs had superior therapeutic effects in the rat model compared with HAL or ALA solutions. The observed therapeutic effects of HAL ESs against P. acnes biofilms and P. acnes–induced inflammation suggest that PDT with HAL-loaded ESs may have potential applications in the treatment of acne. |
| Databáze: | OpenAIRE |
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