Improving sensitivity in simultaneous determination of copper carboxylates by nonaqueous capillary electrophoresis
| Autor: | Pirkko-Leena Laamanen, Rafael Cela, E. Blanco, Rose Matilainen |
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| Rok vydání: | 2006 |
| Předmět: |
Bromides
Analyte Potassium Compounds Carboxylic Acids chemistry.chemical_element Ethylenediaminetetraacetic acid Buffers Sensitivity and Specificity Biochemistry Analytical Chemistry Electrolytes chemistry.chemical_compound Capillary electrophoresis Organometallic Compounds Chelation Carboxylate Edetic Acid Chelating Agents Chromatography Formamides Chemistry Potassium bromide Organic Chemistry Nitrilotriacetic acid Electrophoresis Capillary General Medicine Hydrogen-Ion Concentration Copper Calibration Water Pollutants Chemical |
| Zdroj: | Journal of Chromatography A. 1110:261-267 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2006.01.091 |
| Popis: | A new method of nonaqueous capillary electrophoresis (NACE) with UV spectrophotometric detection was developed and optimized for the simultaneous determination of seven carboxylates (trans-1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic acid (CDTA), diethylenetriaminepentaacetic acid (DTPA), ethylenediaminetetraacetic acid (EDTA), N-(2-hydroxyethyl)ethylenediamine-N,N′,N′-triacetic acid (HEDTA), nitrilotriacetic acid (NTA), 1,3-diaminopropane-N,N,N′,N′-tetraacetic acid (PDTA) and triethylenetetraaminehexaacetic acid (TTHA)) as copper complexes. The method development was carried out by using a fused silica capillary. Background electrolyte (BGE) was optimized and the best separation achieved by using 30 mmol L−1 potassium bromide in N-methylformamide (NMF) at apparent pH (pHapp) 10.2. A voltage of +30 kV and direct UV detection at 280 nm were used in all measurements. Large-volume sample stacking using the electroosmotic flow pump (LVSEP) was tested in addition to basic capillary electrophoresis (CE) and observed to improve the separation of the analyte zones in the capillary. All the peaks in the electropherograms were properly separated, the calibration plots gave excellent correlation coefficients (R2 ≥ 0.994) and all seven copper carboxylate complexes were detected in less than 20 min using both the basic measurements and the large-volume sample stacking method. The new NACE method was tested with lake water and proved to be reliable. |
| Databáze: | OpenAIRE |
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