Development of a quantitative real-time RT-PCR assay for detecting Taiwan ferret badger rabies virus in ear tissue of ferret badgers and mice

Autor: Chung-Hsi Chou, Yu-Hua Shih, Chun-Hsien Tseng, Florence Cliquet, Kuo-Jung Tsai, Wen-Jane Tu, Re-Shang Chen, Yi-Ta Lu, Ai-Ping Hsu, Hsiang-Jung Tsai
Přispěvatelé: Council of Agriculture, National Taiwan University [Taiwan] (NTU), Laboratoire de la rage et de la faune sauvage de Nancy (LRFSN), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)
Rok vydání: 2018
Předmět:
0301 basic medicine
Badger
Rabies
040301 veterinary sciences
[SDV]Life Sciences [q-bio]
030106 microbiology
Taiwan
real-time RT-PCR
medicine.disease_cause
Virus
0403 veterinary science
Mice
03 medical and health sciences
rabies diagnosis
Virology
biology.animal
Mustelidae
medicine
Ferret
Animals
Phylogeny
Taiwan ferret badger rabies
Mice
Inbred BALB C
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
Full Paper
General Veterinary
biology
Reverse Transcriptase Polymerase Chain Reaction
Rabies virus
Ferrets
RNA
04 agricultural and veterinary sciences
Ferret-badger
medicine.disease
biology.organism_classification
3. Good health
Reverse transcription polymerase chain reaction
Real-time polymerase chain reaction
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
RNA
Viral
Zdroj: The Journal of Veterinary Medical Science
Journal of Veterinary Medical Science
Journal of Veterinary Medical Science, Japanese Society of Veterinary Science, 2018, 80 (6), pp.1012-1019. ⟨10.1292/jvms.17-0539⟩
ISSN: 1347-7439
0916-7250
Popis: International audience; In 2013, the first case of Taiwan ferret badger rabies virus (RABV-TWFB) infection was reported in Formosan ferret badgers, and two genetic groups of the virus were distinguished through phylogenetic analysis. To detect RABV-TWFB using a sensitive nucleic acid-based method, a quantitative real-time reverse transcription polymerase chain reaction targeting the conserved region of both genetic groups of RABV-TWFB was developed. This method had a limit of detection (LOD) of 40 RNA copies/reaction and detected viral RNA in brain and ear tissue specimens of infected and dead Formosan ferret badgers and mice with 100% sensitivity and specificity. The mean viral RNA load detected in the ear tissue specimens of ferret badgers ranged from 3.89 × 108 to 9.73 × 108 RNA copies/g-organ, which was 111-fold to 2,220-fold lower than the concentration detected in the brain specimens, but 2,000-fold to 5,000-fold higher than the LOD of the assay. This highly sensitive technique does not require facilities or instruments complying with strict biosafety criteria. Furthermore, it is efficient, safe, and labor-saving as only ear specimens need be sampled. Therefore, it is a promising technique for epidemiological screening of Taiwan ferret badger rabies.
Databáze: OpenAIRE
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