Heterotrimeric G proteins interact with defense-related receptor-like kinases in Arabidopsis
| Autor: | María Nieves Aranda-Sicilia, David Chakravorty, Yuelin Zhang, Yuri Trusov, Natsumi Maruta, José Ramón Botella |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
Physiology G protein Arabidopsis Plant Science Biology Protein Serine-Threonine Kinases Bioinformatics 01 natural sciences Protein–protein interaction 03 medical and health sciences Bimolecular fluorescence complementation Gene Expression Regulation Plant Heterotrimeric G protein 030304 developmental biology G protein-coupled receptor 0303 health sciences G protein-coupled receptor kinase Arabidopsis Proteins fungi Heterotrimeric GTP-Binding Proteins Cell biology G beta-gamma complex Signal transduction Agronomy and Crop Science 010606 plant biology & botany Signal Transduction |
| Zdroj: | Journal of plant physiology. 188 |
| ISSN: | 1618-1328 |
| Popis: | Heterotrimeric G proteins (G-proteins) are versatile signaling elements conserved in Eukaryotes. In animals G-proteins relay signals from 7-transmembrane spanning G protein-coupled receptors (GPCRs) to intracellular downstream effectors; however, the existence of GPCRs in plants is controversial. Contrastingly, a surplus of receptor-like kinases (RLKs) provides signal recognition at the plant cell surface. It is established that G proteins are involved in plant defense and suggested that they relay signals from defense-related RLKs. However, it is unclear how the signaling is conducted, as physical interaction between the RLKs and G proteins has not been demonstrated. Using yeast split-ubiquitin system and Bimolecular Fluorescence Complementation assays, we demonstrate physical interaction between the Gα, Gγ1 and Gγ2 subunits, and the defense-related RD-type receptor like kinases CERK1, BAK1 and BIR1. At the same time, no interaction was detected with the non-RD RLK FLS2. We hypothesize that G-proteins mediate signal transduction immediately downstream of the pathogenesis-related RLKs. |
| Databáze: | OpenAIRE |
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