Novel Extracellular Electron Transfer Channels in a Gram-Positive Thermophilic Bacterium
| Autor: | Mohamed Y. El-Naggar, Elizaveta A. Bonch-Osmolovskaya, Orianna Bretschger, Jonathan R. Lloyd, Vladimir Popov, Sahand Pirbadian, Kar Man Leung, Tamara V. Tikhonova, N. I. Dergousova, Maksim Zakhartsev, Frank T. Robb, Sergey N. Gavrilov, D. G. Zavarzina, David Knight, Ivan M. Elizarov |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
lcsh:QR1-502 iron reduction Microbiology lcsh:Microbiology Electron transfer chemistry.chemical_compound Ferrihydrite Extracellular electrogenesis Magnetite Original Research chemistry.chemical_classification biology Thermophile Electron acceptor Gram-positive bacteria biology.organism_classification chemistry thermophilic prokaryotes Biophysics cardiovascular system multiheme cytochromes lipids (amino acids peptides and proteins) biogenic magnetite crystals Bacteria Archaea |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 11 (2021) Gavrilov, S N, Zavarzina, D G, Elizarov, I M, Tikhonova, T V, Dergousova, N I, Popov, V O, Lloyd, J R, Knight, D, El-naggar, M Y, Pirbadian, S, Leung, K M, Robb, F T, Zakhartsev, M V, Bretschger, O & Bonch-osmolovskaya, E A 2021, ' Novel Extracellular Electron Transfer Channels in a Gram-Positive Thermophilic Bacterium ', Frontiers in Microbiology, vol. 11 . https://doi.org/10.3389/fmicb.2020.597818 |
| Popis: | Biogenic transformation of Fe minerals, associated with extracellular electron transfer (EET), allows microorganisms to exploit high-potential refractory electron acceptors for energy generation. EET-capable thermophiles are dominated by hyperthermophilic archaea and Gram-positive bacteria. Information on their EET pathways is sparse. Here, we describe EET channels in the thermophilic Gram-positive bacterium Carboxydothermus ferrireducens that drive exoelectrogenesis and rapid conversion of amorphous mineral ferrihydrite to large magnetite crystals. Microscopic studies indicated biocontrolled formation of unusual formicary-like ultrastructure of the magnetite crystals and revealed active colonization of anodes in bioelectrochemical systems (BESs) by C. ferrireducens. The internal structure of micron-scale biogenic magnetite crystals is reported for the first time. Genome analysis and expression profiling revealed three constitutive c-type multiheme cytochromes involved in electron exchange with ferrihydrite or an anode, sharing insignificant homology with previously described EET-related cytochromes thus representing novel determinants of EET. Our studies identify these cytochromes as extracellular and reveal potentially novel mechanisms of cell-to-mineral interactions in thermal environments. |
| Databáze: | OpenAIRE |
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