Semiautomated methods for cancellous bone histomorphometry using a general-purpose video image analysis system
| Autor: | P. Ruegg, J.-M. Zhu, W. E. Huffer, R. B. Lepoff |
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| Rok vydání: | 1994 |
| Předmět: |
Reproducibility
Histology Pixel Staining and Labeling Osteoid Magnification Mineralogy Tracing Thresholding Bone and Bones Pathology and Forensic Medicine Perimeter medicine.anatomical_structure Calcification Physiologic Microscopy Fluorescence medicine Image Processing Computer-Assisted Humans Bone Remodeling Cancellous bone Geology Biomedical engineering |
| Zdroj: | Journal of microscopy. 173(Pt 1) |
| ISSN: | 0022-2720 |
| Popis: | SUMMARY Semiautomated methods are used to measure elongated, curved and complex branching profiles and isolated perimeter segments in monochrome video images with a general-purpose analysis system. These methods are used to make the major primary measurements of bone histomorphometry. Accuracy and reproducibility of the image acquisition, processing and measurement system is documented by measuring a semicircular standard of known dimensions. Semiautomated applications of the Ar/Le method for measuring areas and perimeters, and calculating lengths and widths of osteoid seams, lengths of mineralization labels and mineral apposition rate, wall width, indirect measurements of eroded, osteoclastic and osteoblastic perimeters without tracing, and measurement of mineralized or total cancellous bone area and perimeter gave values comparable to measurements of the same parameters by tracing or grid counting techniques with equal or better reproducibility and much greater efficiency. Intraindividual variation in measuring multiple bone biopsies was comparable to that reported with current standard methods. Major sources of variability for semiautomated methods were image magnification and selection of profile edges by thresholding, and sources of variability for manual methods are image magnification, numbers of orthogonal intercepts, tracing speed and accuracy of the algorithm used to measure traced pixels. Semiautomated methods are accurate, reproducible and rapid methods suitable for bone histomorphometry. |
| Databáze: | OpenAIRE |
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