Synergistic action of SPI-1 gene expression in Salmonella enterica serovar typhimurium through transcriptional crosstalk with the flagellar system

Autor:	Christopher V. Rao, Selwan Hamed, Philip D. Aldridge, Xiaoyi Wang, Mohamed Emara, Riham M. Shawky
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	Salmonella typhimurium Microbiology (medical) animal diseases Gene regulatory network lcsh:QR1-502 Acetates Biology Microbiology lcsh:Microbiology 03 medical and health sciences Bacterial Proteins Salmonella Gene expression Gene Transcription factor Crosstalk Regulation of gene expression 0303 health sciences SPI-1 Acetate 030306 microbiology Drug Synergism Gene Expression Regulation Bacterial Nutrients biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Pathogenicity island Cell biology Gene regulation Crosstalk (biology) Salmonella enterica Flagella Trans-Activators bacteria Bistability Signal Transduction Research Article
Zdroj:	BMC Microbiology, Vol 19, Iss 1, Pp 1-12 (2019) BMC Microbiology
ISSN:	1471-2180
DOI:	10.1186/s12866-019-1583-7
Popis:	Background Salmonella enterica serovar Typhimurium is a common food-borne pathogen. S. enterica uses a type III secretion system encoded within Salmonella pathogenicity island 1 (SPI-1) to invade intestinal epithelial cells. A complex network of interacting transcription factors regulates SPI-1 gene expression. In addition, SPI-1 gene expression is coupled to flagellar gene expression. Both SPI-1 and flagellar gene expression are bistable, with co-existing populations of cells expressing and not expressing these genes. Previous work demonstrated that nutrients could be used to tune the fraction of cells expressing the flagellar genes. In the present study, we tested whether nutrients could also tune the fraction of cells expressing the SPI-1 genes through transcriptional crosstalk with the flagellar genes. Results Nutrients alone were not found to induce SPI-1 gene expression. However, when the cells were also grown in the presence of acetate, the concentration of nutrients in the growth medium was able to tune the fraction of cells expressing the SPI-1 genes. During growth in nutrient-poor medium, acetate alone was unable to induce SPI-1 gene expression. These results demonstrate that acetate and nutrients synergistically activate SPI-1 gene expression. The response to acetate was governed by the BarA/SirA two-component system and the response to nutrients was governed by transcriptional crosstalk with the flagella system, specifically through the action of the flagellar regulator FliZ. Conclusions Acetate and nutrients are capable of synergistically activating SPI-1 gene expression. In addition, these signals were found to tune the fraction of cells expressing the SPI-1 genes. The governing mechanism involves transcriptional crosstalk with the flagellar gene network. Collectively, these results further our understanding of SPI-1 gene regulation and provide the basis for future studies investigating this complex regulatory mechanism. Electronic supplementary material The online version of this article (10.1186/s12866-019-1583-7) contains supplementary material, which is available to authorized users.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24aa1337bdbbfdee93907d8e7ca8f9fb http://link.springer.com/article/10.1186/s12866-019-1583-7 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.