Anti-reflection cover to control acoustic intensity in in vitro low-intensity ultrasound stimulation of cells
| Autor: | Meysam Majnooni, Elise Doveri, Jeanne Baldisser, Vincent Long, Julien Houles, Jean-Claude Scimeca, David Momier, Carine Guivier-Curien, Philippe Lasaygues, Cécile Baron |
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| Přispěvatelé: | Institut des Sciences du Mouvement Etienne Jules Marey (ISM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Mécanique et d'Acoustique [Marseille] (LMA ), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Centre de Physique des Particules de Marseille (CPPM), Aix Marseille Université (AMU)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Institut de Recherche sur les Phénomènes Hors Equilibre (IRPHE) |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Acta Acustica Acta Acustica, 2022, 6 (11), ⟨10.1051/aacus/2022007⟩ |
| ISSN: | 2681-4617 |
| DOI: | 10.1051/aacus/2022007 |
| Popis: | Low-intensity ultrasound stimulation is a technique used in therapeutic ultrasound for bone regeneration. However, the underlying mechanisms are still poorly understood. In vitro studies on cell cultures are implemented to understand the processes involved. To analyze the effects of ultrasonic waves on cells, the control of the delivered acoustic intensity is essential. However, depending on the insonification protocol chosen, multiple reflections and standing waves that form inside the culture medium strongly hinder the estimates. In this work, we propose the development and the experimental validation of an anti-reflection cover. We demonstrate that this custom-designed device is effective in avoiding multiple reflections and makes it possible to artificially replace the layer of culture medium with a large amount of water. Finally, an analytical study of the acoustic intensity delivered to the cells is proposed. |
| Databáze: | OpenAIRE |
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