Formononetin induces the mitochondrial apoptosis pathway in prostate cancer cells via downregulation of the IGF-1/IGF-1R signaling pathway
| Autor: | Yu Ye, Zhen-Zhao Li, Xing Zhang, Ling-Yun Bi, Wen-Jun Huang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Pharmacology
Chemistry Growth factor medicine.medical_treatment Pharmaceutical Science General Medicine medicine.disease Molecular biology Prostate cancer chemistry.chemical_compound Complementary and alternative medicine Downregulation and upregulation Apoptosis Drug Discovery Cancer cell medicine Molecular Medicine Formononetin MTT assay Signal transduction |
| Zdroj: | Pharmaceutical Biology. 52:466-470 |
| ISSN: | 1744-5116 1388-0209 |
| Popis: | Context: Formononetin, an isoflavone, can inhibit the proliferation of cancer cells, including those of the prostate. However, its antitumor mechanism remains unclear. Aim: To investigate whether the insulin-like growth factor 1 (IGF-1)/insulin-like growth factor 1 receptor (IGF-1 R) signaling pathway mediates the formononetin antitumor effect on prostate cancer cells. Materials and methods: The viability of PC-3 cells was measured by MTT assay 48 h after formononetin treatment (25, 50 and 100 μM). Formononetin-induced cell apoptosis was measured by Hoechst 33258 staining and flow cytometry. Expression of Bax mRNA was detected by real-time PCR, and the expression levels of Bax and IGF-1 R proteins were detected by western blots. Results: At concentrations12.5 μM, formononetin significantly inhibited the proliferation of human prostate cancer cells. Formononetin increased Bax mRNA and protein expression levels and decreased the expression levels of pIGF-1 R protein in a dose-dependent manner. Conclusion: High concentrations of formononetin-induced apoptosis in androgen-independent prostate cancer cells through inhibition of the IGF-1/IGF-1 R pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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