Tomato root microbiota and Phytophthora parasitica-associated disease

Autor: Benoit Industri, Corinne Rancurel, Marie Larousse, Catherine Etienne, Xavier Nesme, Eric Galiana, Camille Syska, Ferran Palero, Marc Bardin
Přispěvatelé: Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Centre d’Estudis Avançats de Blanes (CEAB-CSIC), Consejo Superior de Investigaciones Científicas, UMR 1355 ISA Institut Sophia Agrobiotech, Université de Nice Sophia-Antipolis (UNSA), Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Station de Pathologie Végétale (AVI-PATHO), Galiana, Eric, Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Unité de Pathologie Végétale (PV), Institut Sophia Agrobiotech [Sophia Antipolis] ( ISA ), Centre National de la Recherche Scientifique ( CNRS ) -Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Institut National de la Recherche Agronomique ( INRA ), Université de Nice - Sophia Antipolis ( UNSA ), Institut National de la Recherche Agronomique ( INRA ), Centre National de la Recherche Scientifique ( CNRS ), Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), Station de Pathologie Végétale ( AVI-PATHO )
Rok vydání: 2017
Předmět:
0301 basic medicine
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
champignon phytopathogène
tomato
Plant Roots
biofilm
tomate
Oomycete
Solanum lycopersicum
host plant
Gene Expression Regulation
Plant
diversité microbienne
RNA
Ribosomal
16S
[ SDV.SA ] Life Sciences [q-bio]/Agricultural sciences
pathologie végétale
Phylogeny
[ SDV.MP.MYC ] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
2. Zero hunger
Rhizosphere
biology
Microbiota
Pseudomonas
High-Throughput Nucleotide Sequencing
food and beverages
phytopathogenic fungus
3. Good health
Agricultural sciences
Mycologie
lcsh:QR100-130
Pseudomonadaceae
Proteobacteria
Metagenomics 16S
rhizosphère
Flavobacteriaceae
phytophthora parasitica
Microbiology (medical)
DNA
Bacterial
Phytophthora
030106 microbiology
Virulence
Mycology
interaction microbienne
Microbiology
DNA
Ribosomal
lcsh:Microbial ecology
metagenomics 16S
03 medical and health sciences
Microbial ecology
microbiote
Symbiosis
Host plant
Plant Diseases
Bacteria
Host (biology)
Research
fungi
Bacteroidetes
Sequence Analysis
DNA
biology.organism_classification
pseudomonas
030104 developmental biology
Biofilms
champignon du sol
Sciences agricoles
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
Microbiome
Microbiome, BioMed Central, 2017, 5:56, ⟨10.1186/s40168-017-0273-7⟩
Microbiome (5:56), . (2017)
Microbiome, BioMed Central, 2017, 5:56, 〈10.1186/s40168-017-0273-7〉
Microbiome, Vol 5, Iss 1, Pp 1-11 (2017)
ISSN: 2049-2618
DOI: 10.1186/s40168-017-0273-7⟩
Popis: Este artículo contiene 11 páginas, 6 figuras.
Background: Interactions between pathogenic oomycetes and microbiota residing on the surface of the host plant root are unknown, despite being critical to inoculum constitution. The nature of these interactions was explored for the polyphagous and telluric species Phytophthora parasitica. Results: Composition of the rhizospheric microbiota of Solanum lycopersicum was characterized using deep re-sequencing of 16S rRNA gene to analyze tomato roots either free of or partly covered with P. parasitica biofilm. Colonization of the host root surface by the oomycete was associated with a shift in microbial community involving a Bacteroidetes/Proteobacteria transition and Flavobacteriaceae as the most abundant family. Identification of members of the P. parasitica-associated microbiota interfering with biology and oomycete infection was carried out by screening for bacteria able to (i) grow on a P. parasitica extract-based medium (ii), exhibit in vitro probiotic or antibiotic activity towards the oomycete (iii), have an impact on the oomycete infection cycle in a tripartite interaction S. lycopersicum-P. parasitica-bacteria. One Pseudomonas phylotype was found to exacerbate disease symptoms in tomato plants. The lack of significant gene expression response of P. parasitica effectors to Pseudomonas suggested that the increase in plant susceptibility was not associated with an increase in virulence. Our results reveal that Pseudomonas spp. establishes commensal interactions with the oomycete. Bacteria preferentially colonize the surface of the biofilm rather than the roots, so that they can infect plant cells without any apparent infection of P. parasitica. Conclusions: The presence of the pathogenic oomycete P. parasitica in the tomato rhizosphere leads to a shift in the rhizospheric microbiota composition. It contributes to the habitat extension of Pseudomonas species mediated through a physical association between the oomycete and the bacteria.
This work was supported by a 3-year grant to Marie Larousse funded by INRA and the PACA region (France), and by a 1-year ATER position funded by the University of Nice Sophia Antipolis. Ferran Palero acknowledges a post-doctoral contract funded by the Beatriu de Pinos Programme of the Generalitat de Catalunya.
Databáze: OpenAIRE
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