Characteristic profile of antibody responses to PPD, ESAT-6, and CFP-10 of Mycobacterium tuberculosis in pulmonary tuberculosis suspected cases in Surabaya, Indonesia

Autor: Ni Made Mertaniasih, Mamiko Niki, Manabu Ato, Fihiruddin, Sohkichi Matsumoto, Soedarsono, Desak Nyoman Surya Suameitria Dewi, Wayan Tunas Artama, Yoshitaka Tateishi, Yuriko Ozeki
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
Interferon gamma release assay
lcsh:QR1-502
lcsh:Microbiology
Reference Values
Antibody response' profile
Child
0303 health sciences
GeneXpert MTB/RIF
biology
Pulmonary tuberculosis
Middle Aged
Infectious Diseases
ESAT-6
Female
NTM
PPD
Adult
Microbiology (medical)
Tuberculosis
Adolescent
Tuberculin
Enzyme-Linked Immunosorbent Assay
Sensitivity and Specificity
complex mixtures
Statistics
Nonparametric
lcsh:Infectious and parasitic diseases
Mycobacterium tuberculosis
Young Adult
03 medical and health sciences
Bacterial Proteins
medicine
Humans
lcsh:RC109-216
Tuberculosis
Pulmonary
Aged
Retrospective Studies
Antigens
Bacterial
CFP-10
Tuberculin Test
030306 microbiology
business.industry
medicine.disease
biology.organism_classification
bacterial infections and mycoses
Indonesia
Case-Control Studies
Antibody Formation
Immunology
Nontuberculous mycobacteria
business
Zdroj: Brazilian Journal of Infectious Diseases, Vol 23, Iss 4, Pp 246-253 (2019)
Brazilian Journal of Infectious Diseases, Volume: 23, Issue: 4, Pages: 246-253, Published: 24 OCT 2019
Brazilian Journal of Infectious Diseases v.23 n.4 2019
Brazilian Journal of Infectious Diseases
Brazilian Society of Infectious Diseases (BSID)
instacron:BSID
ISSN: 1413-8670
Popis: Accurate and rapid diagnostic tools are important aspects of managing tuberculosis (TB) cases appropriately. However, the sensitivity and specificity of diagnostic kits based on immune response such as the tuberculin skin test (TST) and interferon gamma release assay (IGRA) are still debated. Thus, the exploration and assessment of specific biomarker-targeted antibodies are needed for the development of an accurate and rapid diagnostic tool. The present study was conducted in patients with a respiratory problem suspected to be TB at Dr. Soetomo Hospital, Surabaya, Indonesia. Among 102 patients tested by GeneXpert and AFB, 59 serum samples were from cases retrospectively determined to have active TB. A total of 102 serum of healthy controls (HC) was also collected. The PPD antigen and the recombinant CFP-10 and ESAT-6 proteins were prepared. Antibody responses against these proteins were evaluated by ELISA. All samples were also screened for the possibility of Mycobacterium avium-intracellulare complex (MAC) infection using Capilla MaC kit. The results showed that TB patients had a significantly higher concentration of IgG antibody in response to PPD than the HC. In addition, the receiver operating characteristic (ROC) curve analysis showed that PPD was acceptable for diagnostic purposes with an AUC value of 0.835 (95% CI 0.770-0.900, p
Databáze: OpenAIRE
Externí odkaz: