Administration of Parathyroid Hormone, Prostaglandin E2, or 1-alpha,25-Dihydroxyvitamin D3 Restores the Bone Inductive Activity of rhBMP-2 in Aged Rats
| Autor: | Izumi Asahina, Yuhji Kabasawa, Ken Omura, Akemi Gunji |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Aging
medicine.medical_specialty Anabolism Injections Subcutaneous Parathyroid hormone chemistry.chemical_element Biology Calcium Bone morphogenetic protein Bone and Bones Dinoprostone Calcitriol Bone Density Internal medicine Genetics medicine Animals Rats Wistar Prostaglandin E2 Growth Substances Molecular Biology Drug Implants Bone Development Dose-Response Relationship Drug Cell Biology General Medicine Alkaline Phosphatase Recombinant Proteins Rats Dose–response relationship Endocrinology chemistry Parathyroid Hormone Bone Morphogenetic Proteins Alkaline phosphatase Female medicine.drug Hormone |
| Zdroj: | DNA and Cell Biology. 22:541-546 |
| ISSN: | 1557-7430 1044-5498 |
| DOI: | 10.1089/104454903322405428 |
| Popis: | Bone morphogenetic protein (BMP) induces bone formation in young rodents, but aging causes a reduction in the bone-forming ability of BMP. Most patients who require bone reconstruction are relatively old. Accordingly, we examined whether anabolic hormones could restore the bone inductive activity of rhBMP-2 in aged rats. rhBMP-2 in a carrier pellet was implanted subcutaneously in both 4- and 50-week-old female Wistar rats. PTH, PGE2, or 1,25(OH)2D3 was injected every day during the period of BMP implantation. The pellets were harvested, and were examined both histologically and biochemically 2 weeks after implantation. Bone-forming ability was measured by alkaline phosphatase (ALP) activity and calcium (Ca) content. Pellets in 50-week-old rats showed a significant reduction in bone formation compared to pellets in 4-week-old rats. However, daily injections of PTH into 50-week-old rats restored both ALP activity (103 +/- 4.6%) and Ca content (105 +/- 2.6%). 1,25(OH)2D3 and PGE2 also restored Ca content (103 +/- 4.5% and 98 +/- 3.8%, respectively) and stimulated ALP activity (142 +/- 2.3% and 133 +/- 3.6%). These results show that the administration of these hormones restores bone-forming ability in aged rats. A combination treatment of these hormones with rhBMP-2 might be applicable to the reconstruction of bone defects in elderly patients. |
| Databáze: | OpenAIRE |
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