Different gene expression in immunoglobulin-mutated and immunoglobulin-unmutated forms of chronic lymphocytic leukemia

Autor: Richard Rosenquist, A. Ferrer, Sakari Knuutila, Juhani Vilpo, Ulf Thunberg, Mauno Vihinen, Bálint Nagy, Gerard Tobin, Juha Ollila, Yan Aalto
Rok vydání: 2004
Předmět:
Male
Cancer Research
Chronic lymphocytic leukemia
DNA Mutational Analysis
Immunoglobulin Variable Region
Biology
Klinikai orvostudományok
Polymerase Chain Reaction
03 medical and health sciences
0302 clinical medicine
hemic and lymphatic diseases
Genetics
medicine
Humans
Molecular Biology
Gene
Aged
Oligonucleotide Array Sequence Analysis
030304 developmental biology
Regulation of gene expression
0303 health sciences
ZAP-70 Protein-Tyrosine Kinase
Genes
Immunoglobulin
Gene Expression Regulation
Leukemic
Gene Expression Profiling
ZAP70
Orvostudományok
Sequence Analysis
DNA
Middle Aged
Protein-Tyrosine Kinases
medicine.disease
Leukemia
Lymphocytic
Chronic
B-Cell
Molecular biology
Neoplasm Proteins
Gene expression profiling
Leukemia
030220 oncology & carcinogenesis
Cancer research
Immunoglobulin heavy chain
Female
PAX5
Immunoglobulin Heavy Chains
Zdroj: Cancer Genetics and Cytogenetics. 153:69-72
ISSN: 0165-4608
DOI: 10.1016/j.cancergencyto.2003.12.016
Popis: The mutation status of the immunoglobulin heavy chain variable regions (IgVH) has been found to be a good prognostic indicator for B-cell chronic lymphocytic leukemia (CLL) because unmutated VH genes are associated with rapid disease progression and shorter survival time. To study the differences in gene expression between the Ig-unmutated and Ig-mutated CLL subtypes, we performed gene expression profiling on 31 CLL cases and investigated the VH gene mutation status by sequencing. The array data showed that the greatest variances between the unmutated (20 cases) and the mutated (11 cases) group were in expressions of ZAP70, RAF1, PAX5, TCF1, CD44, SF1, S100A12, NUP214, DAF, GLVR1, MKK6, AF4, CX3CR1, NAFTC1, and HEX. ZAP70 was significantly more expressed in the Ig-unmutated CLL group, whereas the expression of all the other genes was higher in the Ig-mutated cases. These results corroborate a recent finding, according to which the expression of ZAP70 can predict the VH mutation status and suggest that RAF1, PAX5, and other differentially expressed genes may offer good markers for differentiating unmutated cases from mutated cases and thus serve as prognostic markers.
Databáze: OpenAIRE
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