Exploring gastric bacterial community in young pigs
| Autor: | Francesca Bertolini, Paolo Trevisi, Vincenzo Motta, Paolo Bosi, Anisa Ribani, Giuseppina Schiavo, Luca Fontanesi |
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| Přispěvatelé: | Motta, Vincenzo, Trevisi, Paolo, Bertolini, Francesca, Ribani, Anisa, Schiavo, Giuseppina, Fontanesi, Luca, Bosi, Paolo |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Swine Physiology lcsh:Medicine Database and Informatics Methods mucosa gastrica RNA Ribosomal 16S Medicine and Health Sciences lcsh:Science Data Management Mammals suini Multidisciplinary Ecology Stomach Gastrointestinal Microbiome Bacterial taxonomy Genomics Body Fluids medicine.anatomical_structure Medical Microbiology Vertebrates Proteobacteria Anatomy Sequence Analysis Research Article Microbial Taxonomy Computer and Information Sciences Bioinformatics 030106 microbiology Sequence Databases Microbial Genomics Biology Research and Analysis Methods Microbiology Microbial Ecology 03 medical and health sciences medicine Gastric mucosa Genetics microbiota Animals Microbiome Stomaco Taxonomy Bacteria Ecology and Environmental Sciences Bacterial Taxonomy lcsh:R Organisms Biology and Life Sciences Bacteriology Ribosomal RNA biology.organism_classification 16S ribosomal RNA Gastrointestinal Tract 030104 developmental biology Biological Databases Gastric Mucosa Amniotes lcsh:Q Digestive System |
| Zdroj: | PLoS ONE, Vol 12, Iss 3, p e0173029 (2017) PLoS ONE |
| Popis: | Microbiota plays an important role in the homeostasis of the gastrointestinal tract. Understanding the variations of the commensal microbiota composition is crucial for a more efficient control of enteric infectious diseases and for the reduction of the use of antibiotics in animal production, which are the main points of interest for improved animal healthcare and welfare and for consumer health protection. Even though the intestinal microbiota has been extensively studied, little is known about the gastric microbiota. This pilot study was aimed at a descriptive analysis of the gastric microbiota in healthy pigs and at the identification of any differences among four potentially distinct microbial niches in the stomach. Gastric mucosal samples from the oxyntic area, the pylorus and the gastric groove, and a sample of gastric contents were collected from four healthy weaned pigs. Bacterial DNA was isolated and extracted from each sample and amplicons from the V6 region of the 16S rRNA gene were sequenced using Ion Torrent PGM. The data were analysed by an "unsupervised" and a "supervised" approach in the Ribosomal Database Project (RDP) pipeline. Proteobacteria was the dominant phylum in all the samples. Differences in bacterial community composition were found between mucosal and content samples (one-way ANOSIM pairwise post hoc test, p < 0.05); instead, the different mucosal regions did not show differences between them. The mucosal samples were characterised by Herbiconiux and Brevundimonas, two genera which include cellulolytic and xylanolytic strains. Nevertheless, additional larger trials are needed to support the data presented in this pilot study and to increase the knowledge regarding the resident microbiota of the stomach. |
| Databáze: | OpenAIRE |
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