Synthesis and characterization of oriented glyco-capturing macroligand
| Autor: | Srinivas, Chalagalla, Yanyang, Wang, Dale, Ray, Xiangqun, Zeng, Indika, Perera, Xue-Long, Sun |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Boron Compounds
Polymers Carbohydrates Biosensing Techniques Biochemistry Polymerization Glycomics chemistry.chemical_compound Organic chemistry Molecular Biology Cyanates chemistry.chemical_classification Binding Sites Ligand Organic Chemistry Polymer Quartz crystal microbalance Combinatorial chemistry Boronic Acids Glycoproteomics chemistry Covalent bond Quartz Crystal Microbalance Techniques Molecular Medicine Boronic acid |
| Zdroj: | Chembiochem : a European journal of chemical biology. 11(14) |
| ISSN: | 1439-7633 |
| Popis: | An oriented glyco-capturing macroligand was synthesized by site-specific immobilization of an O-cyanate chain-end-functionalized boronic acid containing polymer (boropolymer) onto an amine surface. The O-cyanate chain-end-functionalized boropolymer was synthesized by arylamine-initiated cyanoxyl-mediated free-radical polymerization in a one-pot fashion. The chain-end O-cyanate was confirmed by (13)C NMR spectroscopy. The specific carbohydrate-binding capacity of the boropolymer was evaluated by an alizarin red S assay. Oriented and covalent immobilization of the O-cyanate chain-end-functionalized boropolymer onto the amine-modified solid surfaces and its specific glyco-capturing capacity were confirmed by the quartz crystal microbalance (QCM) and atomic force microscopy (AFM) techniques. The oriented multivalent glyco-capturing ligand can be used for efficient carbohydrate and glycoconjugate purification and identification, and thus is expected to constitute a core strategy of glycomics and glycoproteomics and carbohydrate-sensing applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text