Immunohistochemical analysis of ghrelin expression in various types of adrenal tumors
| Autor: | Barbara Bromińska, Agnieszka Malinska, Marek Ruchała, Hanna Komarowska, Marcin Rucinski, Zhanat Komekbai, Katarzyna Bednarek-Rajewska |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Histology Enteroendocrine cell Pheochromocytoma Pathology and Forensic Medicine Adrenocortical adenoma Internal medicine Adrenal Glands medicine Biomarkers Tumor Adrenocortical carcinoma Humans Adrenal cortex business.industry digestive oral and skin physiology General Medicine medicine.disease Immunohistochemistry Neoplasms Neuroepithelial Ghrelin medicine.anatomical_structure Endocrinology ROC Curve Adrenocortical Adenoma Adenocarcinoma Adrenal medulla business hormones hormone substitutes and hormone antagonists |
| Zdroj: | Folia histochemica et cytobiologica. 59(2) |
| ISSN: | 1897-5631 |
| Popis: | Introduction. Ghrelin, originally isolated from the endocrine cells of the gastric mucosa, is also expressed in many peripheral tissues, including normal adrenals and adrenocortical tumors. It was shown that ghrelin stimulates proliferation and inhibits apoptosis of adrenocortical cells. In the current study, we compared ghrelin expression at the protein level in various adrenal tumors. We analyzed whether immunoreactive ghrelin could be considered as a potential marker for different types of adrenal tumors. Material and methods. Study was carried out on 200 adrenal specimens arranged on microscope slide in tissue microarray format. We performed standardized immunohistochemical reactions with semiquantitative reaction intensity measurements. Results. At the protein level, the expression of ghrelin was significantly reduced in adrenocortical adenocarcinoma in relation to the control group and pheochromocytoma as well as cancer-adjacent normal adrenal tissue. In contrast, a relatively high ghrelin expression was found in pheochromocytoma compared to all analyzed groups, with the exception of cancer-adjacent normal adrenal tissue. Conclusions. The ghrelin expression profile at the protein level may be associated with the type of adrenal tumor. In this context, our results suggest that adrenal immunoreactive ghrelin may be considered as a sensitive and specific marker for differentiating adrenocortical carcinoma from adrenocortical adenoma and pheochromocytoma. |
| Databáze: | OpenAIRE |
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