Cloning and functional expression of a human orthologue of rat vanilloid receptor-1
| Autor: | Paul R. Murdock, Graham D Smith, Isro S Gloger, John Terrett, Kathryn Ellington, D.Malcolm Duckworth, Andrew D. Medhurst, Amanda J. L. Barton, Mark H Harries, Christopher D. Benham, Martin J. Gunthorpe, Andrew D. Randall, Helen J. Meadows, Simon Topp, David C. Harrison, Rab K. Prinjha, William Cairns, Catherine E. Clarke, Gareth J. Sanger, Owen Jenkins, John B. Davis, Philip David Hayes |
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| Rok vydání: | 2000 |
| Předmět: |
Genotype
Receptors Drug Xenopus Molecular Sequence Data Resiniferatoxin TRPV1 TRPV Cation Channels Biology Molecular cloning Chromosomes Cell Line chemistry.chemical_compound Complementary DNA Gene expression Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular Cloning Polymorphism Genetic Base Sequence Temperature Nociceptors DNA Hydrogen-Ion Concentration Molecular biology Rats Anesthesiology and Pain Medicine Neurology chemistry Capsaicin Expression cloning Oocytes lipids (amino acids peptides and proteins) Neurology (clinical) |
| Zdroj: | Pain. 88:205-215 |
| ISSN: | 0304-3959 |
| Popis: | Capsaicin, resiniferatoxin, protons or heat have been shown to activate an ion channel, termed the rat vanilloid receptor-1 (rVR1), originally isolated by expression cloning for a capsaicin sensitive phenotype. Here we describe the cloning of a human vanilloid receptor-1 (hVR1) cDNA containing a 2517 bp open reading frame that encodes a protein with 92% homology to the rat vanilloid receptor-1. Oocytes or mammalian cells expressing this cDNA respond to capsaicin, pH and temperature by generating inward membrane currents. Mammalian cells transfected with human VR1 respond to capsaicin with an increase in intracellular calcium. The human VR1 has a chromosomal location of 17p13 and is expressed in human dorsal root ganglia and also at low levels throughout a wide range of CNS and peripheral tissues. Together the sequence homology, similar expression profile and functional properties confirm that the cloned cDNA represents the human orthologue of rat VR1. |
| Databáze: | OpenAIRE |
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