The N-terminus of the Montano virus nucleocapsid protein possesses broadly cross-reactive conformation-dependent epitopes conserved in rodent-borne hantaviruses
| Autor: | María de Lourdes Romero-Almaraz, Takaaki Koma, Ikuo Takashima, Cornelio Sánchez-Hernández, Hiroaki Kariwa, Jiro Arikawa, Takahiro Seto, Ngonda Saasa, Kumiko Yoshimatsu, Kenta Shimizu, Celso Ramos, Takahiro Sanada, Haruka Yoshida, Kentaro Yoshii |
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| Jazyk: | angličtina |
| Předmět: |
Monoclonal antibody
Orthohantavirus Immunogen Hantavirus Infections animal diseases viruses Amino Acid Motifs Molecular Sequence Data Montano virus Sequence alignment Biology Cross Reactions medicine.disease_cause Antibodies Viral Cross-reactivity Epitope Conserved sequence Cell Line Rodent Diseases Epitopes Mice Antibody Specificity Virology medicine Animals Humans Amino Acid Sequence Peptide sequence Conserved Sequence Hantavirus Nucleocapsid protein Mice Inbred BALB C Antibodies Monoclonal virus diseases Nucleocapsid Proteins Sequence Alignment Conformational epitope |
| Zdroj: | Virology. (1):48-57 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2012.03.001 |
| Popis: | The hantavirus nucleocapsid (N) protein is an important immunogen that stimulates a strong and cross-reactive immune response in humans and rodents. A large proportion of the response to N protein has been found to target its N-terminus. However, the exact nature of this bias towards the N-terminus is not yet fully understood. We characterized six monoclonal antibodies (mAbs) against the N protein of Montano virus (MTNV), a Mexican hantavirus. Five of these mAbs recognized eight American hantaviruses and six European and Asian hantaviruses, but not the Soricomorpha-borne Thottapalayam hantavirus. The N protein-reactive binding regions of the five mAbs were mapped to discontinuous epitopes within the N-terminal 13–51 amino acid residues, while a single serotype-specific mAb was mapped to residues 1–25 and 49–75. Our findings suggest that discontinuous epitopes at the N-terminus are conserved, at least in rodent-borne hantaviruses, and that they contribute considerably to N protein cross-reactivity. |
| Databáze: | OpenAIRE |
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