| Autor: |
Yasunori Totsuka, Kuniaki Suzuki, Kanchu Tei, Yoshitaka Yoshimura, Nadia Galal, Waleed R. El-Beialy, Yoshiaki Deyama |
| Rok vydání: |
2009 |
| Předmět: |
|
| Zdroj: |
Molecular Medicine Reports. 3 |
| ISSN: |
1791-2997 |
| DOI: |
10.3892/mmr_00000226 |
| Popis: |
Proteomic and genomic studies commonly involve the assessment of mRNA levels using reverse transcription-polymerase chain reaction (PCR) and real-time quantitative PCR. An internal standard RNA is fundamentally analyzed along with the investigated mRNA to document the specificity of the effect(s) on mRNA and to correct for inter-sample variations. In our studies implementing estrogen treatments on different cell lines, we initially used glyceraldehyde-3-phosphate dehydrogenase (G3PDH) as an internal standard. However, the results of PCR amplification demonstrated that 17β-estradiol enhanced the expression of the G3PDH gene, rendering it impossible to use G3PDH as an unbiased comparative control. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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