Modularly Assembled Upconversion Nanoparticles for Orthogonally Controlled Cell Imaging and Drug Delivery
| Autor: | Swati Shikha, Muthu Kumara Gnanasammandhan Jayakumar, Yong Zhang, Xiang Zheng, Zhen Zhang, Yi Zhang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Imagination
Materials science Chemical substance Paclitaxel Cell Survival media_common.quotation_subject Nanoparticle Antineoplastic Agents Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Drug Delivery Systems Humans Molecule General Materials Science Particle Size Fluorescent Dyes media_common Doping 021001 nanoscience & nanotechnology Fluorescence Photon upconversion 0104 chemical sciences Microscopy Fluorescence Drug delivery Nanoparticles 0210 nano-technology HeLa Cells |
| Zdroj: | ACS Applied Materials & Interfaces. 12:12549-12556 |
| ISSN: | 1944-8252 1944-8244 |
| Popis: | Upconversion nanoparticles (UCNPs) have been used effectively as light transducers to convert near-infrared irradiation to short-wavelength emissions for photoactivation in deep tissues. UCNPs with single/multiple emissions under excitation at a single wavelength can be used for simultaneous activation of single or multiple photosensitive molecules only; an ideal multifunctional UCNP nanoplatform should not only have the ability to load multiple molecules but also should activate them at the right time with the right dose when necessary, depending upon the application for which it is used. The control of many biological processes requires complex (simultaneous or subsequent) photoactivation at different time points. Subsequent photoactivation requires UCNPs with orthogonal fluorescence emissions, which can be controlled independently. So far, there are only a few reports about UCNPs with orthogonal emissions. Synthesis of these orthogonal emission nanoparticles is complicated and tedious because nanoparticles with multiple shells need to be synthesized, and different lanthanide ions need to be doped into different shells. Also, there is no flexibility for changing the doped ions and emission profile after the nanoparticles are produced. Here, we have demonstrated a versatile method to modularly assemble individual UCNPs into UCNP clusters (UCNPs-C) with adjustable emissions. The synthesis is much easier, and there is a lot of flexibility in changing the particle size, shape, doped ions, and emission profile. We have demonstrated the use of such UCNPs-C for color encoding at the nanoscale. We further designed orthogonal photoactivatable UCNPs-C (OP-UCNPs-C), which can be independently activated under 980 nm excitation for red emission and 808 nm excitation for UV/blue emission. These OP-UCNPs-C were used for independent activation of processes for cell imaging (980 nm) and drug delivery (808 nm). In comparison to the traditional nonprogrammed activation, a programmed controlled imaging and drug delivery process could guarantee highly targeted and enhanced cell death of cancerous cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|