Inhibitory effect of Thai propolis on human osteoclastogenesis
| Autor: | Suttichai Krisanaprakornkit, Phumisak Louwakul, Thanapat Sastraruji, Anupong Makeudom, Pattama Chailertvanitkul, Chayarop Supanchart, Nattaporn Wimolsantirungsri |
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| Rok vydání: | 2018 |
| Předmět: |
biology
Chemistry Acid phosphatase 030209 endocrinology & metabolism 030206 dentistry Propolis Peripheral blood mononuclear cell Resorption Andrology 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Osteoclast biology.protein medicine Periodontal fiber Viability assay Oral Surgery Tartrate-resistant acid phosphatase |
| Zdroj: | Dental traumatology : official publication of International Association for Dental Traumatology. |
| ISSN: | 1600-9657 |
| Popis: | BACKGROUND/AIM Avulsed teeth should be immediately replanted into the socket or otherwise kept in a physiologic storage medium to maintain periodontal ligament cell viability. A previous study has demonstrated that Thai propolis extract can maintain viability of human periodontal ligament cells. However, root resorption by osteoclasts often occurs when the avulsed teeth are replanted. The aim of this study was to determine the inhibitory effect of Thai propolis extract on human osteoclastogenesis in vitro. MATERIALS AND METHODS Human peripheral blood mononuclear cells were isolated for osteoclast precursors and cultured in the presence or absence of various non-toxic concentrations of propolis extract, as determined by the alamarBlue® assay, during in vitro induction of osteoclastogenesis. Osteoclast formation was examined by tartrate-resistant acid phosphatase staining, actin ring formation, and real-time polymerase chain reaction. The resorption pit assay was performed to determine osteoclast function. RESULTS Non-toxic concentrations of propolis extract suppressed osteoclast formation by significantly decreasing the percentages of tartrate-resistant acid phosphatase-positive multinuclear cells and the ratios of cells with F-actin ring formation (P |
| Databáze: | OpenAIRE |
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