Diversity and dynamics of lactic acid bacteria in Atole agrio, a traditional maize-based fermented beverage from South-Eastern Mexico, analysed by high throughput sequencing and culturing
| Autor: | Judith Espinosa, Alba Pérez-Cataluña, Dolores Reyes, Rosa Aznar, Carmen Wacher, Purificación Carrasco, Patricia Elizaquível |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Weissella Lactococcus 030106 microbiology Biology Microbiology Acetobacteraceae Beverages 03 medical and health sciences Lactobacillales RNA Ribosomal 16S Leuconostoc Food science Mexico Molecular Biology Computational Biology High-Throughput Nucleotide Sequencing food and beverages Biodiversity General Medicine biology.organism_classification Fermentation Food Microbiology Pediococcus Leuconostocaceae Lactobacillus plantarum |
| Zdroj: | Antonie van Leeuwenhoek. 111:385-399 |
| ISSN: | 1572-9699 0003-6072 |
| Popis: | The purpose of this work was to analyse the diversity and dynamics of lactic acid bacteria (LAB) throughout the fermentation process in Atole agrio, a traditional maize based food of Mexican origin. Samples of different fermentation times were analysed using culture-dependent and -independent approaches. Identification of LAB isolates revealed the presence of members of the genera Pediococcus, Weissella, Lactobacillus, Leuconostoc and Lactococcus, and the predominance of Pediococcus pentosaceus and Weissella confusa in liquid and solid batches, respectively. High-throughput sequencing (HTS) of the 16S rRNA gene confirmed the predominance of Lactobacillaceae and Leuconostocaceae at the beginning of the process. In liquid fermentation Acetobacteraceae dominate after 4 h as pH decreased. In contrast, Leuconostocaceae dominated the solid fermentation except at 12 h that were overgrown by Acetobacteraceae. Regarding LAB genera, Lactobacillus dominated the liquid fermentation except at 12 h when Weissella, Lactococcus and Streptococcus were the most abundant. In solid fermentation Weissella predominated all through the process. HTS determined that Lactobacillus plantarum and W. confusa dominated in the liquid and solid batches, respectively. Two oligotypes have been identified for L. plantarum and W. confusa populations, differing in a single nucleotide position each. Only one of the oligotypes was detected among the isolates obtained from each species, the biological significance of which remains unclear. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink