Response of Cytokines and Hydrogen Peroxide to Sporothrix schenckii Exoantigen in Systemic Experimental Infection
| Autor: | Amanda Costa Gonçalves, Lucas Souza Ferreira, Danielle Cardoso Geraldo Maia, Alexander Batista-Duharte, Francine Alessandra Manente, Deivys Leandro Portuondo, Iracilda Zeppone Carlos, José Carlos Rebuglio Vellosa, Marisa Campos Polesi |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Estadual de Ponta Grossa (UEPG), Medical Science University |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Antigens Fungal Macrophage Veterinary (miscellaneous) Interleukin-1beta 030106 microbiology Pro-inflammatory cytokines Virulence Spleen Apoptosis Nitric Oxide S. schenckii Applied Microbiology and Biotechnology Microbiology Proinflammatory cytokine Mice 03 medical and health sciences Medical microbiology medicine Animals Sporothrix schenckii biology Sporotrichosis business.industry Sporothrix Hydrogen Peroxide Macrophage Activation biology.organism_classification medicine.disease Hydrogen peroxide Interleukin-10 medicine.anatomical_structure Immunology Macrophages Peritoneal Interleukin-2 Exoantigen business Agronomy and Crop Science |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2018-12-11T17:01:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-04-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The response of hydrogen peroxide (H2O2) and cytokines during an experimental sporotrichosis in male Swiss mice was assessed over a period of 10 weeks by monitoring macrophage activation challenged with exoantigen (ExoAg) from the fungus Sporothrix schenckii. The studied endpoints were: H2O2 production, fungal burden at spleen, apoptosis in peritoneal macrophages, and IL-1β, IL-6, IL-2, IL-10 production. During the two first weeks of infection was observed low burden of yeast in spleen and high response of H2O2, IL-2, and IL-1β. The weeks of highest fungal burden (fourth–sixth) coincided with major apoptosis in peritoneal macrophages, normal production of IL-6 and lower production of H2O2, IL-2, and IL-1β, suggesting a role for these three last in the early control of infection. On the other hand, IL-1β (but not IL-6) was recovered since the sixth week, suggesting a possible role in the late phase of infection, contributing to the fungal clearance in conjunction with the specific mechanisms. The IL-10 was elevated until the sixth, principally in the second week. These results evidences that ExoAg is involved in the host immune modulation, influencing the S. Schenckii virulence, and its role is related with the time of the infection in the model used. Departamento de Análises Clínicas Faculdade de Ciências Farmacêuticas de Araraquara Universidade Estadual Paulista “Júlio de Mesquita Filho” - UNESP, Rua Expedicionários do Brasil n° 1621 Departamento de Análises Clínicas e Toxicológicas Setor de Ciências Biológicas e da Saúde Universidade Estadual de Ponta Grossa – UEPG Immunotoxicology Laboratory Toxicology and Biomedicine Center (TOXIMED) Medical Science University, Autopista Nacional Km. 1 1/2 Departamento de Análises Clínicas Faculdade de Ciências Farmacêuticas de Araraquara Universidade Estadual Paulista “Júlio de Mesquita Filho” - UNESP, Rua Expedicionários do Brasil n° 1621 FAPESP: 2009/11442-9 |
| Databáze: | OpenAIRE |
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