Isolation of Shiga Toxin-ProducingEscherichia colifrom Ground Beef Using Multiple Combinations of Enrichment Broths and Selective Agars
| Autor: | Gerardo Anibal Leotta, Pablo Piñeyro, Lucía Galli, Luciano Héctor Linares, Victoria Brusa, Nora Lía Padola, Emanuel E. Ortega |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
food.ingredient Otras Ciencias Biológicas 030106 microbiology Biology Beef cattle medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Tryptic soy broth Shiga Toxin Foodborne Diseases Ciencias Biológicas 03 medical and health sciences chemistry.chemical_compound food medicine Animals Agar Food microbiology Food science Escherichia coli Escherichia coli Infections Shiga-Toxigenic Escherichia coli Ciencias Veterinarias GROUND BEEF Shiga toxin Isolation (microbiology) Culture Media Red Meat chemistry ISOLATION SHIGA TOXIN-PRODUCING ESCHERICHIA COLI Food Microbiology biology.protein bacteria Cattle Animal Science and Zoology ENRICHMENT MacConkey agar CIENCIAS NATURALES Y EXACTAS Food Science |
| Zdroj: | Foodborne Pathogens and Disease. 13:163-170 |
| ISSN: | 1556-7125 1535-3141 |
| DOI: | 10.1089/fpd.2015.2034 |
| Popis: | Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens, and beef cattle are recognized as the principal reservoir. The aims of this study were (1) to identify the most sensitive combination of selective enrichment broths and agars for STEC isolation in artificially inoculated ground beef samples, and (2) to evaluate the most efficient combination(s) of methods for naturally contaminated ground beef samples. A total of 192 ground beef samples were artificially inoculated with STEC and non-stx bacterial strains. A combination of four enrichment broths and three agars were evaluated for sensitivity, specificity, and positive predictive value for STEC isolation from experimentally inoculated samples. Enrichments with either modified tryptic soy broth (mTSB) containing 8mg/L novobiocin (mTSB-8) or modified Escherichia coli (mEC) broth followed by isolation in MacConkey agar were the most sensitive combinations for STEC isolation of artificially inoculated samples. Independently, both enrichments media followed by isolation in MacConkey were used to evaluate ground beef samples from 43 retail stores, yielding 65.1% and 58.1% stx-positive samples by RT-PCR, respectively. No difference was observed in the isolate proportions between these two methods (8/25 [32%] and 8/28 [28.6%]). Identical serotypes and stx genotypes were observed in STEC strains isolated from the same samples by either method. In this study, no single enrichment protocol was sufficient to detect all STEC in artificially inoculated samples and had considerable variation in detection ability with naturally contaminated samples. Moreover, none of the single or combinations of multiple isolation agars used were capable of identifying all STEC serogroups in either artificially inoculated or naturally occurring STEC-contaminated ground beef. Therefore, it may be prudent to conclude that there is no single method or combination of isolation methods capable of identifying all STEC serogroups. Instituto de Genética Veterinaria Comisión de Investigaciones Científicas de la provincia de Buenos Aires |
| Databáze: | OpenAIRE |
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